This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ABCB1 antibody (M00049) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
Filter:
IHC analysis of ABCB1 using anti-ABCB1 antibody (M00049).
ABCB1 was detected in a paraffin-embedded section of human adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB1 Antibody (M00049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ABCB1 was detected in a paraffin-embedded section of human adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB1 Antibody (M00049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody
ABCB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
ABCB1 antibody was positively detected by IHC in:
Rat heart tissue, human cardiac muscle tissue, Mouse skin tissue, Rat spleen tissue, human adrenal gland tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Rat
Recommend
Tested
Recommend
Human
Recommend
Tested
Recommend
Mouse
Recommend
Tested
Recommend
Product Information
Product Name
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody
View all ABCB1 Antibodies
View all ABCB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FDB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human P Glycoprotein
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
180 kDa
Calculated Molecular Weight
141.5 kDa
Cite This Product
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00049)
Protocols
Immunohistochemistry (IHC) protocol for ABCB1 antibody
Publications
Application:
Species:
Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel
Published: 2022-08-25
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: WB
Impact Factor: 6.51
Species: Zebrafish
PMID: 36029990
HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway
Published: 2020-05-15
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 3.26
Species: Rat
PMID: 32417325
Hydroxychloroquine reverses the drug resistance of leukemic K562/ADM cells by inhibiting autophagy
Published: 2019-08-27
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Human
PMID: 31485616
Review
Have you used Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026