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Product Info Summary
| SKU: | M00049 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00049
BM4508 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody catalog # M00049. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00049)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FDB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human P Glycoprotein
Reactive Species
M00049 is reactive to ABCB1 in Human, Mouse, Rat
Observed Molecular Weight
180 kDa
Calculated molecular weight
141.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00049 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
IHC: Rat heart tissue, human cardiac muscle tissue, Mouse skin tissue, Rat spleen tissue, human adrenal gland tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of P Glycoprotein expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:1000 dilution.
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IHC analysis of ABCB1 using anti-ABCB1 antibody (M00049).
ABCB1 was detected in a paraffin-embedded section of human adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB1 Antibody (M00049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of ABCB1 using anti-ABCB1 antibody (M00049).
ABCB1 was detected in a paraffin-embedded section of human cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB1 Antibody (M00049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat spleen, using the Antibody at 1:1000 dilution.
Specific Publications For Anti-P Glycoprotein ABCB1 Rabbit Monoclonal Antibody (M00049)
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