Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

Boster Bio Anti-P-GlycoProtein (MDR) ABCB4 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: MA1060
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

See all Abcb1a products

SKU/Catalog Number

MA1060

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P-GlycoProtein (MDR) ABCB4 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

F4

Isotype

Mouse IgG1

Immunogen

Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1060 is reactive to ABCB4 in Human

Applications

MA1060 is guaranteed for IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ABCB4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P21447

Gene ID

18671

Gene Name

ABCB4

Full Name

ATP-dependent translocase ABCB1

Weight

140.647kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-dependent translocase ABCB1 Abcb1a|Abcb4, Evi, Evi32, Mdr, Mdr1a, Mdr3, P, P-, P-gp, Pgp, Pgy, Pgy-, Pgy-3, Pgy3, mdr-, mdr-3|ATP-binding cassette, sub-family B (MDR/TAP), member 1A|ATP-dependent translocase ABCB1|ATP-binding cassette sub-family B member 1A|ATP-binding cassette, sub-family B (MDR/TAP), member 4|ATP-binding cassette, subfamily B, member 1A|P-glycoprotein 3|ecotropic viral integration site 32|multi-drug resistance 3|multidrug resistance protein 3|multiple drug resistant 1a|phospholipid transporter ABCB1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ABCB4, check out the ABCB4 Infographic

ABCB4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ABCB4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1060 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gao S, Liu Q, Wang X, Lin B, Zhang S. Med Oncol. 2010 Sep;27(3):960-7. Doi: 10.1007/S12032-009-9317-6. Epub 2009 Sep 23. Effects Of Lewis Y Antigen On The Gene Expression Of Multiple Drug Resistance-Associated Proteins In Human Ovarian Cancer Rmg-...

Wang X, Deng R, Lu Y, Xu Q, Yan M, Ye D, Chen W. Basic Clin Pharmacol Toxicol. 2013 Jan;112(1):25-33. Doi: 10.1111/J.1742-7843.2012.00921.X. Epub 2012 Aug 22. Gambogic Acid As A Non-Competitive Inhibitor Of Atp-Binding Cassette Transporter B1 Reve...

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6 Customer Q&As for Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

Question

See attached the WB image, lot number and protocol we used for brain blood vessel using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

I was wanting to use your anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) for IHC for human brain blood vessel on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain blood vessel identification?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

You can see on the product datasheet, MA1060 anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain blood vessel in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-05

Question

My question regarding product MA1060, anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1060 anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-30

Question

We are currently using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

The anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-12

Question

Is a blocking peptide available for product anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060)?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

I was wanting to use to test anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 on human brain blood vessel for research purposes, then I may be interested in using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Carter

Verified customer

Asked: 2017-05-30

Answer

The products we sell, including anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-30

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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MA1060
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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