SKU MA1060
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number F4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)
SKU/Catalog Number MA1060
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P21447
Gene Name Abcb1a
Protein Name Multidrug resistance protein 1A
Tissue Specificity ABCB1: Expressed in liver, kidney, small intestineand brain.
Alternative Names Multidrug resistance protein 1A;3.6.3.44;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Molecular Weight 140647 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .
Research Areas Atpases, Cancer, Lipid Metabolism, Metabolism, Nuclear Import / Export, Plasma Membrane, Protein Trafficking, Signal Transduction

*You can search these to find other products in these research areas.
Background P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.

Other Recommended Resources

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Order Product (MA1060)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.

Troubleshooting

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Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

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Publications

Gao S, Liu Q, Wang X, Lin B, Zhang S. Med Oncol. 2010 Sep;27(3):960-7. Doi: 10.1007/S12032-009-9317-6. Epub 2009 Sep 23. Effects Of Lewis Y Antigen On The Gene Expression Of Multiple Drug Resistance-Associated Proteins In Human Ovarian Cancer Rmg-...
Wang X, Deng R, Lu Y, Xu Q, Yan M, Ye D, Chen W. Basic Clin Pharmacol Toxicol. 2013 Jan;112(1):25-33. Doi: 10.1111/J.1742-7843.2012.00921.X. Epub 2012 Aug 22. Gambogic Acid As A Non-Competitive Inhibitor Of Atp-Binding Cassette Transporter B1 Reve...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to abcb1 antibody, mdr1 antibody, p gp antibody