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Product Info Summary
| SKU: | MA1060 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4)
SKU/Catalog Number
MA1060
BM0508 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P-GlycoProtein (MDR) ABCB1 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
F4
Isotype
Mouse IgG1
Immunogen
Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1060 is reactive to ABCB1 in Human
Observed Molecular Weight
200 kDa
Calculated molecular weight
141.5 kDa
Background of ABCB1
P-Glycoprotein,, also known as Multidrug Resistance 1 (MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1060 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Positive Control
WB: human DLD1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ABCB1 using anti-ABCB1 antibody (MA1060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human DLD1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ABCB1 antigen affinity purified monoclonal antibody (Catalog # MA1060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ABCB1 at approximately 200 kDa. The expected band size for ABCB1 is at 141 kDa.
Specific Publications For Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4) (MA1060)
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Customer Q&As
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6 Customer Q&As for Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4)
Question
See attached the WB image, lot number and protocol we used for brain blood vessel using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) for IHC for human brain blood vessel on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain blood vessel identification?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can see on the product datasheet, MA1060 anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain blood vessel in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-05
Question
My question regarding product MA1060, anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1060 anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-30
Question
We are currently using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
Question
Is a blocking peptide available for product anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) (MA1060). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use to test anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 on human brain blood vessel for research purposes, then I may be interested in using anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Carter
Verified customer
Asked: 2017-05-30
Answer
The products we sell, including anti-P-GlycoProtein(MDR) antibody (Monoclonal, F4) MA1060, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-30
