|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)|
|Immunogen||Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.|
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Multidrug resistance protein 1A|
|Tissue Specificity||ABCB1: Expressed in liver, kidney, small intestineand brain.|
|Alternative Names||Multidrug resistance protein 1A;188.8.131.52;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Molecular Weight||140647 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .|
|Background||P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.|
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