|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)|
|Description||Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) F4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)|
|Replacement Item||This antibody may replace the following items: sc-13131|sc-1517|sc-1517-R|sc-55510|sc-71557|sc-73354|sc-8313 from Santa Cruz Biotechnology.|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Multidrug resistance protein 1|
|Molecular Weight||140647 MW|
|Protein Function||Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .|
|Tissue Specificity||ABCB1: Expressed in liver, kidney, small intestineand brain.|
|Sequence Similarities||Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Alternative Names||Multidrug resistance protein 1A;220.127.116.11;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;|
|Research Areas||signal transduction|metabolism|mitochondrial|pathways and processes|mitochondrial metabolism|mitochondrial markers||
Background for Multidrug resistance protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.