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- Table of Contents
ABCB1 antibody (A00049-1) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of U20S cells using anti-P Glycoprotein antibody (A00049-1).
Overlay histogram showing U20S cells stained with A00049-1 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein Antibody (A00049-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control."
Overlay histogram showing U20S cells stained with A00049-1 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein Antibody (A00049-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control."
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Anti-P Glycoprotein/ABCB1 Antibody Picoband®
ABCB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
ABCB1 antibody was positively detected by WB in:
human THP-1 whole cell, human A375 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCB1 antibody was positively detected by ICC/IF in:
U20S cell
ABCB1 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-P Glycoprotein/ABCB1 Antibody Picoband®
View all ABCB1 Antibodies
View all ABCB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, which shares 62.9% amino acid (aa) sequence identity with rat P Glycoprotein.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated Molecular Weight
141.5 kDa
Background of ABCB1
P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.
Cite This Product
Anti-P Glycoprotein/ABCB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00049-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Sheep
83%
Moderate Homology
Mouse
80%
Moderate Homology
Rabbit
80%
Moderate Homology
Cow
77%
Moderate Homology
Dog
77%
Moderate Homology
Rat
71%
Moderate Homology
Zebrafish
40%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ABCB1 antibody
Flow Cytometry (FC) protocol for ABCB1 antibody
Publications
Application:
Species:
Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance
Published: 2023-08-22
Journal: ACS Nano
Application: IF
Impact Factor: 17.10
Species: Mouse
PMID: 37606317
Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel
Published: 2022-08-25
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: WB
Impact Factor: 6.51
Species: Zebrafish
PMID: 36029990
A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells
Published: 2022-08-12
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 6.21
Species: Human
PMID: 36012254
A 20(S)-protopanoxadiol derivative overcomes multi-drug resistance by antagonizing ATP-binding cassette subfamily B member 1 transporter function
Published: 2016-01-25
Journal: Oncotarget
Application: WB
Impact Factor: 5.01
Species: Human
PMID: 26824187
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Published: 2016-01-01
Journal: Theranostics
Application: IF
Impact Factor: 8.85
Species: Human,Rat
PMID: 26877779
The mechanism of the opening of the blood–brain barrier by borneol: A pharmacodynamics and pharmacokinetics combination study
Published: 2013-10-25
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF
Impact Factor: 2.76
Species: Rat
Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes
Published: 2013-04-06
Journal: TUMOR BIOLOGY
Application: WB
Impact Factor: 2.52
Species: Human
PMID: 23564482
Expression and significance of hypoxia-inducible factor-1 alpha and MDR1/P-glycoprotein in human colon carcinoma tissue and cells
Published: 2010-03-09
Journal: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Application: IF
Impact Factor: 2.26
Species: Human
PMID: 20217131
Growth retardation of fetal rats exposed to nicotine in utero: Possible involvement of CYP1A1, CYP2E1, and P-glycoprotein
Published: 2008-04-28
Journal: ENVIRONMENTAL TOXICOLOGY
Application: IHC
Impact Factor: 1.73
Species: Rat
PMID: 18442069
Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis
Published: 2004-03-14
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.95
Species: Human
PMID: 15149863
Overexpression of ZNRD1 Promotes Multidrug-Resistant Phenotype of Gastric Cancer Cells Through Upregulation of P-Glycoprotein
Published: 2004-02-02
Journal: CANCER BIOLOGY & THERAPY
Application: WB
Impact Factor: 3.02
Species: Human
PMID: 14726695
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Product Promise
We promise Anti-P Glycoprotein/ABCB1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00049-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026