Product Info Summary
SKU: | A00049 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ABCB1/Mdr1 Antibody
View all MDR1/ABCB1 Antibodies
SKU/Catalog Number
A00049
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ABCB1/Mdr1 Antibody (Catalog # A00049). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-ABCB1/Mdr1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00049)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00049 is reactive to ABCB1 in Human
Applications
A00049 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
141.479kDa
Background of MDR1/ABCB1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: User optimized
WB: 1:200 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ABCB1 expression in crude membrane extracts from HF insect cells. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. ABCB1 was detected using rabbit anti-ABCB1 Antigen Affinity purified polyclonal antibody (Catalog # A00049) at 1:600. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Click image to see more details
ABCB1 was detected in paraffin-embedded sections of human kidney tissue using rabbit anti-ABCB1 Antigen Affinity purified polyclonal antibody (Catalog # A00049) at 20 μg/mL. Image provided courtesy of Lifespan Biosciences.
Protein Target Info & Infographic
Gene/Protein Information For ABCB1 (Source: Uniprot.org, NCBI)
Gene Name
ABCB1
Full Name
ATP-dependent translocase ABCB1
Weight
141.479kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC20; ABCB1; ABCB1B; ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CD243 antigen; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; EC 3.6.3; EC 3.6.3.44; GP170; IBD13; MDR1; MDR1MGC163296; multidrug resistance protein 1; P-glycoprotein 1; P-gp; PGY1; PGY1P-GP ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170 ATP binding cassette subfamily B member 1 ATP-dependent translocase ABCB1|ATP-binding cassette, sub-family B (MDR/TAP), member 1|P-glycoprotein 1|colchicin sensitivity|doxorubicin resistance|multidrug resistance protein 1|phospholipid transporter ABCB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCB1, check out the ABCB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCB1/Mdr1 Antibody (A00049)
Hello CJ!
A00049 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Breast Cancer Risk–Associated SNPs in the mTOR Promoter Form De Novo KLF5- and ZEB1-Binding Sites that Influence the Cellular Response to Paclitaxel
Tetrandrine and fangchinoline, bisbenzylisoquinoline alkaloids from Stephania tetrandra can reverse multidrug resistance by inhibiting P-glycoprotein activity in multidrug resistant human cancer cells
Chen Q, Deng X,Hu X,Guan S,He M,Wang Y,Wei B,Zhang J,Zhao H,Yao W,Jin F,Liu Y,Chen J,Olapade OI,Wu H,Wei M.Breast Cancer Risk-Associated SNPs in the mTOR Promoter Form De Novo KLF5- and ZEB1-Binding Sites that Influence the Cellular Response to Paclitaxel.Mol Cancer Res.2019 Nov;17(11):2244-2256.doi:10.1158/1541-7786.MCR-18-1072.Epub 2019 Aug 29.PMID:31467112.
Species: Human,Mouse
A00049 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000
Sheng X,Li Y,Li Y,Liu W,Lu Z,Zhan J,Xu M,Chen L,Luo X,Cai G,Zhang S.PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics.BMC Cancer.2019 Aug 27;19(1):840.doi:10.1186/s12885-019-6029-y.PMID:31455288; PMCID:PMC6712771.
Species: Human,Mouse
PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics Xiaoli Sheng, et al. BMC Cancer. 2019; 19: 840. Published online 2019 Aug 27. doi: 10.1186/s12885-019-6029-y
A 20(S)-protopanoxadiol derivative overcomes multi-drug resistance by antagonizing ATP-binding cassette subfamily B member 1 transporter function
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