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Product Info Summary
| SKU: | A00049 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ABCB1/Mdr1 Antibody
SKU/Catalog Number
A00049
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ABCB1/Mdr1 Antibody (Catalog # A00049). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ABCB1/Mdr1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00049)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00049 is reactive to ABCB1 in Human
Calculated molecular weight
141.5 kDa
Background of ABCB1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00049 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: User optimized
WB: 1:200 - 1:2,000
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot.
Validation Images & Assay Conditions
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IHC using Boster ABCB1 Antibody at 20 ug/ml against FFPE human kidney. Moderate to strong cytoplasmic and membranous staining seen in this example. Similar staining also observed in other tissues including liver, kidney, and small intestine. The antibody showed minimal background staining and worked well in formalin-fixed tissues. Image provided courtesy of Lifespan Biosciences.
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Western blot using Boster's affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
Specific Publications For Anti-ABCB1/Mdr1 Antibody (A00049)
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