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ABCB1 antibody (A00049-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Flow Cytometry analysis of U20S cells using anti-P Glycoprotein/ABCB1 antibody (A00049-3).
Overlay histogram showing U20S cells stained with A00049-3 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A00049-3 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-P Glycoprotein/ABCB1 Antibody Picoband®
ABCB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
ABCB1 antibody was positively detected by WB in:
rat PC-12 whole cell, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCB1 antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ABCB1 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-P Glycoprotein/ABCB1 Antibody Picoband®
View all ABCB1 Antibodies
View all ABCB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human P Glycoprotein/ABCB1 recombinant protein (Position: R669-Y710).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
140 kDa
Calculated Molecular Weight
141.5 kDa
Background of ABCB1
P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.
Cite This Product
Anti-P Glycoprotein/ABCB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00049-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Sheep
83%
Moderate Homology
Cow
79%
Moderate Homology
Mouse
74%
Moderate Homology
Rabbit
71%
Moderate Homology
Dog
71%
Moderate Homology
Rat
69%
Low homology
Zebrafish
36%
Low homology
Drosophila
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCB1 antibody
Immunohistochemistry (IHC) protocol for ABCB1 antibody
Flow Cytometry (FC) protocol for ABCB1 antibody
Publications
Application:
Species:
Time-Resolved Quantification of Single-Cell Extracellular Vesicles Secretion for Drug Resistance Evaluation
Published: 2025-07-14
Journal: ANALYTICAL CHEMISTRY
Impact Factor: 6.70
Species: Human
PMID: 40658044
Gastric cancer mesenchymal stem cells promote tumor glycolysis and chemoresistance by regulating B7H3 in gastric cancer cells
Published: 2024-01-16
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.00
Species: Human
PMID: 38226525
Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel
Published: 2022-08-25
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: WB
Impact Factor: 6.51
Species: Zebrafish
PMID: 36029990
Regulation of P-glycoprotein efflux activity by Z-guggulsterone of Commiphora mukul at the blood-brain barrier
Published: 2016-02-20
Journal: JOURNAL OF THE NEUROLOGICAL SCIENCES
Application: WB
Impact Factor: 2.13
Species: Rat
PMID: 27000241
In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier
Published: 2013-10-22
Journal: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
Application: WB
Impact Factor: 2.69
Species: Rat
PMID: 24161469
Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats
Published: 2013-02-26
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 2.03
Species: Rat
PMID: 23600383
Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles
Published: 2012-01-17
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: WB
Impact Factor: 3.35
Species: Human
PMID: 22274587
Various doses of fractioned irradiation modulates multidrug resistance 1 expression differently through hypoxia-inducible factor 1α in esophageal cancer cells
Published: 2011-09-01
Journal: DISEASES OF THE ESOPHAGUS
Application: WB
Impact Factor: 1.54
PMID: 21309917
Design and Implementation of Electrochemical Cytosensor for Evaluation of Cell Surface Carbohydrate and Glycoprotein
Published: 2010-04-06
Journal: ANALYTICAL CHEMISTRY
Impact Factor: 5.21
PMID: 20369831
Glutathione depletion upregulates P-glycoprotein expression at the blood-brain barrier in rats
Published: 2010-01-08
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 1.74
Species: Rat
PMID: 19505374
Indole-3-carbinol alters placental cytochrome P450 1A1 and P-glycoprotein levels in rats: A potential role in intensifying fetal intrauterine growth-retardation produced by tobacco smoke
Published: 2006-06-27
Journal: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
Impact Factor: 0.78
Species: Rat
PMID: 16806864
Regulation of Multidrug Resistance by MGr1-Antigen in Gastric Cancer Cells
Published: 2005-12-30
Journal: TUMOR BIOLOGY
Application: WB
Impact Factor: 2.35
Species: human
PMID: 16340247
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Product Promise
We promise Anti-P Glycoprotein/ABCB1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00049-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026