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- Table of Contents
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3 Citations 13 Q&As
Facts about Prolactin.
Human | |
---|---|
Gene Name: | PRL |
Uniprot: | P01236 |
Entrez: | 5617 |
Belongs to: |
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somatotropin/prolactin family |
PRL; Prolactin
Mass (kDA):
25.876 kDA
Human | |
---|---|
Location: | 6p22.3 |
Sequence: | 6; NC_000006.12 (22287246..22302897, complement) |
Secreted.
If you want to find out the most effective uses of the PRL marker are, then you'll have to read this article. This article will provide information on how to use the marker in ELISA and IHC as well as qPCR and expression of mRNA. We will then discuss the most popular uses for the PRL marker in different applications. You'll also learn about how to use this antibody in various settings.
To perform IHC staining with the PRL, you will first choose the appropriate chromogenic substrate. DAB and HRP are the most common combinations. A positive control will be a section of tissue which is known to express the protein of interest. A tissue section that does not express the antigen of interest will be the negative control. It is crucial to apply the correct counterstain fluorescent IHC because the chromogen must not interfere with signals of the labeling for reporter.
There are four forms of PRL that can be expressed in humans and mice. The most well-known isoform of PRL is human PRL that is expressed in the skin. IHC staining can reveal the PRL marker that is a fluorescent protein. To confirm the positive labeling of PRL in human prostate cancer, we need to follow the same procedure as in mice. The PRL marker is expressed in four forms in mice, and three in humans.
PRL staining can be used to identify basal cell clusters. To determine the degree of PRL expression, we utilized the PRL marker as well as CK-5/CK-8 stains. To distinguish between high and low scores PRL staining, the PRL staining results were analysed using paired t tests. All tests had a significance threshold of 0.05. We conducted the analyses using GraphPad Prism version 6.00 for Windows.
The ELISA using the Boster Bio PRM marker reacts with Human Prolactin. The monoclonal antibody can be purchased in liquid form in PBS or 50 percent glycerol. You can also purchase an peptide that blocks the immune system to use as an immunogen. Blocking peptides are priced differently according to the length of the immunogen as well as the antibody.
The HESCs isolated from the nPTB or sPE were grown for 13 days. In these studies, the cells of patients did not respond to decidualization. They also did not respond to antibodies specific for macrophages or hematopoietic cells. The results of the ELISA were similar to the similar results of a previous study. The cells that were isolated from the new study showed an F-actin-like pattern arrangement that is typical of the fibroblast-like cells.
The results were confirmed using qRT-PCR. Four genes were examined to determine their relative expression. A control gene (b-actin) was used as an internal control. Each gene had specific primers: 5'-agatgtgttgttgttgtgttgttg-aggactt-gtt-ggactt-aggactt-gtttg-tagat-ag.
Many studies have shown that the peptide was akin to the N-protein. It also evoked similar antibody responses to the entire N-protein. It was also observed that this peptide binds to the entire N-protein. This makes it a great candidate for the ELISA. The peptide can be utilized as an immunoassay marker detect bioPRL antibodies against human anti-Boster. antibodies.
Boster Bio's Anti-Prolactin PRL Monoclonal Antibody reacts with Human, Mouse, or Rat Caveolin-1 proteins. This antibody is available in liquid form, which includes 0.5% BSA. Blocking peptides can be purchased separately at different prices depending on their lengths as well as their immunogen specificity. If you are looking to determine which antibody to use take a look at some examples of the most beneficial uses of PRL markers.
PRL is a member the PTP superfamily. It encodes a ribonucleoprotein that has an catalytic domain. PRL-3 carries the CX5R signature PTP active sequence. This protein is found in many human cancers, including metastatic types. The stage and progression of cancer is closely linked to PRL-3 expression. The impact of emodin PRL-3 expression is unclear.
PRL can be used in many areas of molecular biology , including the analysis of mRNA expression. The cellular component of PRL is essential for the analysis of the expression of mRNA. In this case it can be used to determine transcription and translation of gene expression. It has been proven that PRL has an effect on the amount of adenosine triphosphate (ATP) mRNA. The protein is also crucial in the analysis of the methylation of RNA.
Trizol was used to extract RNA from cells. 0.5 mg of transcript was reverse-transcribed into cDNA. The cDNA was then quantified by QPCR using an applied Biosystems 7500 Real Time PCR System. The primers used were 5'-forward and 5'-reverse to identify GAPDH, PRL-3. The reaction volume was 20ml. To normalize the mRNA data, GAPDH was used as the control gene.
The Boster Microplate Reader is compatible with 0.5 to 1 Ml aqueous solutions and has a 450nm wavelength for in detecting PRL. Multichannel pipettes are recommended for larger quantities. Researchers can utilize the Boster Bio PRL Detection kit to test for ATF4 and RUNX2 inside cells.
Immunohistochemistry using the PRL marker is done on paraffin-fixed formalin-fixed tissues. The sections were deparaffinized and then incubated in 3% H2O2 to block endogenous peroxidase activity. The anti-PRL-3 antibody, from Santa Cruz Biotechnology, was used for staining. Maixin Biotechnology offered the MaxVisionTM HRP-Polymer anti-Mouse IHC kit.
The PRL-3 protein is expressed in all cancer cells, including stroma. PRL-3 expression was found in five out of six cases. PRL-3 expression also was detected in a tumor that had metastasized, but it was mainly residual. Although it was not a significant expression, the PRL-3 protein was related to several aspects of cancer biology including the presence or the stage of cancer cells.
Since the PRL protein is the hallmark of cancer, this IHC kit for PRL is an excellent diagnostic tool. This test is not without its difficulties. First, labels have to be applied to the antibodies. In other words, it is important to use the antibodies that have different fluorescent spectra in order to ensure that the results are reliable. Furthermore, the antibodies should not cause significant brown staining.
The PRL protein is located in the liver of many patients with colorectal cancer. The PRL protein was first identified in colorectal metastases. It has been associated with poor prognosis. It is linked to a variety of types malignant tumors, including gastric, colorectal and the ovarian cancer.
Boster Bio Human Prolactin ELIISA kit is built on sandwich ELISA technology. Precoated 96-well plates are used to prepare the test sample as well as an anti-prolactin mouse monoclonal antibody. Then, the test sample and the standards are added. A goat biotinylated detection polyclonal antibody is added to the wells, followed by an Avidin-Biotin-Peroxidase complex. Finally, conjugates that aren't bound are flushed away using PBS or TBS buffer.
Boster Bio's Human Prolactin ELISA Kit was created by Steven Xia, a renowned histologist. It has high affinity and sensitivity at the picogram level. Boster Bio antibodies are tested and validated in a transparent manner for a variety of applications such as Flow Cytometry and IHC. Boster Bio backs their products with a lifetime warranty and technical assistance. Each month, they expand their product range.
PMID: 6260780 by Cooke N.E., et al. Human prolactin. cDNA structural analysis and evolutionary comparisons.
PMID: 6325171 by Truong A.T., et al. Isolation and characterization of the human prolactin gene.
*More publications can be found for each product on its corresponding product page