Human Prolactin ELISA Kit PicoKine™

Human Prolactin ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0593
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, citrate).

Product Name

Human Prolactin ELISA Kit PicoKine™

See all Prolactin products

SKU/Catalog Number

EK0593

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Prolactin ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Prolactin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0593)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L29-C227

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0593 is reactive to PRL in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, citrate).

Application Guarantee

EK0593 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0593-CAP Anti-Human PRL Pre-coated 96-well strip microplate 1
EK0593-ST Human PRL Standard 2 vials, 10 ng/tube
EK0593-DA Human PRL Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0270.1230.2170.4090.7801.2371.5531.933

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Prolactin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2781989470627419285020
Standard deviation18.0795.47338.8321.64129.17371.48
CV (%)6.5%4.8%7.2%7.9%6.7%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Prolactin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127826632329929121.687.4%
Sample 21989189219441862192148.662.5%
Sample 347064695505849264846153.083.1%
*number of samples for each test n=16.

Gene/Protein Information For PRL (Source: Uniprot.Org, NCBI)

Uniprot ID

P01236

Gene ID

5617

Gene Name

PRL

Full Name

Prolactin

Weight

25.876kDa

Superfamily

somatotropin/prolactin family

Alternative Names

PRL; Prolactin PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRL, check out the PRL Infographic

PRL infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0593 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chronic endometritis modifies decidualization in human endometrial stromal cells

Garrido-Gomez T, Dominguez F, Qui?onero A, Diaz-Gimeno P, Kapidzic M, Gormley M, Ona K, Padilla-Iserte P, McMaster M, Genbacev O, Perales A, Fisher SJ, Sim

Xia Y, Qi F, Zou J, Yao Z. Brain Res. 2014 Dec 10;1592:34-43. Influenza A(H1N1) Vaccination During Early Pregnancy Transiently Promotes Hippocampal Neurogenesis And Working Memory. Involvement Of Th1/Th2 Balance.

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6 Customer Q&As for Human Prolactin ELISA Kit PicoKine™

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Prolactin in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-05-30

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Prolactin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-05-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

T. Dayal

Verified customer

Asked: 2019-12-08

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-12-08

Question

Q: how to proceed with the analysis of ELISA data? I have performed Prolactin level in plasma.

W. Campbell

Verified customer

Asked: 2019-11-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-25

Question

Q: Can Prolactin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

K. Babu

Verified customer

Asked: 2019-06-15

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-06-15

Question

Can EK0593 detect rhesus monkey prolactin?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

We have not used EK0593 with Rhesus Monkey samples. The sequence homology with rhesus monkey is 98%. https://www.uniprot.org/blast/uniprot/B201812033707B37BB34871DC742D8A439176A1E10305B6U We are uncertain if the kit will detect rhesus monkey prolactin. So,we suggest that a pilot test is ran with EK0593.

Boster Scientific Support

Answered: 2018-08-09

Question

Q: is it okay to use EDTA plasma as samples in Human Prolactin Picokine® ELISA Kit (Catalog # EK0593)?

W. Krishna

Verified customer

Asked: 2017-01-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Prolactin causing disruption of its protein structure. Prolactin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Prolactin ELISA, treating samples with various anticoagulants and decided that heparin or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-01-26

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