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- Table of Contents
Prl antibody (PB9411) performs well in ELISA, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Western blot analysis of Prolactin using anti-Prolactin antibody (PB9411).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human Resistin Protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin antigen affinity purified polyclonal antibody (Catalog # PB9411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin at approximately 10 kDa. The expected band size for Prolactin is at 10 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human Resistin Protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin antigen affinity purified polyclonal antibody (Catalog # PB9411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin at approximately 10 kDa. The expected band size for Prolactin is at 10 kDa.
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Anti-Prolactin/PRL Antibody Picoband®
Prl antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, WB |
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, -
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Prolactin/PRL Antibody Picoband®
View all PRL Antibodies
View all PRL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Prolactin recombinant protein (Position: L30-C226). Mouse Prolactin shares 59.8% and 84.8% amino acid (aa) sequence identity with human and rat Prolactin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
10 kDa
Calculated Molecular Weight
25.5 kDa
Background of Prl
Prolactin (PRL) also known as luteotropic hormone (LTH) is a protein that in humans is encoded by the PRL gene. Prolactin is a peptide hormone discovered by Henry Friesen. Although it is perhaps best known for its role in lactation, prolactin already existed in the oldest known vertebrates-fishes-where its most important functions were probably related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Cite This Product
Anti-Prolactin/PRL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9411)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Publications
Application:
Species:
Xianglong Meng,Chenzi Lyu,Junnan Ma,Xiaoyan Zhang,Cong Hu,Xiaojuan Su,Chenxu Ning,Wenbin Xie,Shuosheng Zhang,"Metabolomics and Network Pharmacology-Based Investigation into the Mechanisms Underlying the Therapeutic Effect of a New Chinese Traditional Medicine (Cui Nai Ling) on Bromocriptine-Induced Hypogalactia",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 8857449,17 pages,2021.https://doi.org/10.1155/2021/8857449
Species: Rat
PMID: -
Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
PMID: 27827961
MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1? induced in human endometriosis stromal cells in vitro
PMID: 24133591
Huang F, Wang H, Zou Y, Liu Q, Cao J, Yin T. Int J Clin Exp Pathol. 2013 Oct 15;6(11):2487-96. Ecollection 2013. Effect Of Gnrh-Ii On The Esc Proliferation, Apoptosis And Vegf Secretion In Patients With Endometriosis In Vitro.
PMID: 24228111
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Product Promise
We promise Anti-Prolactin/PRL Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9411
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026