SKU RP1021
Size 100μg/vial
Reactivity Mouse
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, WB

Overview

Product Name Anti-Prolactin Antibody
SKU/Catalog Number RP1021
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Prolactin(PRL) detection. Tested with WB, ELISA in Mouse.
Cite This Product Anti-Prolactin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1021)
Host Rabbit
Immunogen E. coli-derived mouse Prolactin recombinant protein(Position: L30-C226).
Reactivity Mouse

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Images And Assay Conditions

/antibody/rp1021 1 WB anti prolactin antibody.jpg

Western blot analysis of Prolactin expression in mouse testis extract (lane 1). Prolactin at 26KD was detected using rabbit anti- Prolactin Antigen Affinity purified polyclonal antibody (Catalog # RP1021) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P06879
Gene Name Prl
Protein Name Prolactin
Alternative Names Prolactin;PRL;Prl;
Subcellular Localization Secreted.
Molecular Weight 25496 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Prolactin acts primarily on the mammary gland by promoting lactation.
Research Areas Growth Factors/Hormones, Lipid Metabolism, Metabolism, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases

*You can search these to find other products in these research areas.
Background Prolactin(PRL) also known as luteotropic hormone(LTH) is a protein that in humans is encoded by the PRL gene. Prolactin is a peptide hormone discovered by Henry Friesen. Although it is perhaps best known for its role in lactation, prolactin already existed in the oldest known vertebrates-fishes-where its most important functions were probably related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

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Order Product (RP1021)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Guaranteed product quality

We promise all of our products perform as described in datasheets.

Customer Reviews

Review by
Rating:

Source: Biocompare.com

"Specific and low variability between lots"

--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST

Applications ELISA
Sample Purified mouse prolactin and plasma
Detection TMB Substrate

"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."

(Posted on 8/23/2016)
Write a review for RP1021

Publications

Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
In vitro apoptosis effects of GnRHII on endometrial stromal cells from patients with endometriosis
Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with endometriosis in vitro
MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1? induced in human endometriosis stromal cells in vitro

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to pr-3 antibody, prolactin receptor antibody