PTGS2 Antibodies

Prostaglandin G/H synthase 2 (PTGS2)

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis (PubMed:26859324, PubMed:27226593). Constitutively expressed in certain tissues in physiological conditions, like the endothelium, kidney and brain, and in pathological conditions, such as in cancer.

PTGS2 accounts for production of inflammatory prostaglandins. Up- regulation of PTGS2 is also correlated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis.

Gene Information

Human
Gene Name:	PTGS2
Uniprot:	P35354
Entrez:	5743

Family

Belongs to:
prostaglandin G/H synthase family

Alternative Names

COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 1.14.99.1; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2

Molecular Weight

Mass (kDA):
68.996 kDA

Genomic Context

Human
Location:	1q31.1
Sequence:	1; NC_000001.11 (186671791..186680423, complement)

Subcellular Localizations

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Diseases

• Malignant Neoplasms
• Neoplasms
• Inflammation
• Carcinoma
• Carcinogenesis
• Tumor Angiogenesis
• Adenocarcinoma
• Pain
• Colorectal Cancer
• Neoplasm Metastasis
• Arthritis
• Malignant Tumor Of Colon
• Cell Transformation, Neoplastic

• Colorectal Neoplasms
• Colonic Neoplasms
• Rheumatoid Arthritis
• Degenerative Polyarthritis
• Pathologic Neovascularization
• Cell Invasion
• Malignant Squamous Cell Neoplasm

Pathways

• Angiogenesis
• Cell Proliferation
• Pathogenesis
• Cell Growth
• Inflammatory Response
• Secretion
• Cell Cycle
• Cell Death
• Localization
• Induction Of Apoptosis
• Immune Response
• Methylation
• Platelet Aggregation

• Parturition
• Excretion
• Ovulation
• Reverse Transcription
• Cell Migration
• Cytokine Production
• Cell Adhesion

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Acetylation
• Oxidation
• Reduction
• Nitration
• Demethylation
• Ubiquitination
• Dephosphorylation

• Glycosylation
• Carboxylation
• Deacetylation
• Hydroxylation
• Amidation
• Biotinylation
• Prenylation
• Acylation
• Phosphorothioation
• Farnesylation

Research Areas

• Cancer
• Cell Biology
• Cellular Markers
• Cytokine Research
• Hypoxia

• Lipid and Metabolism
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PTGS2

Best Uses Of The PTGS2 Marker

PTGS2 is a monoclonal antibody that detects prostaglandins that cause inflammation. This antibody is utilized in ELISA and RT-PCR kits. Here is an overview of its application in a range of applications. Learn more about the features of the PTGS2 marker. Also, learn more about uses of this antibody. One of the many monoclonal antibodies that are available is the PTGS2 marker.

PTGS2 monoclonal antibody

PTGS2 is a monoclonic antibody derived from mice immunized using recombinant proteins. It is available in PBS that contains 0.09 percent sodium azide. The purification process is performed using protein G columns and dialysis on PBS. To avoid freezing-thaw cycles, it should be kept at 2-8 degrees C in portions. Suitable for in vitro diagnostics and research, PTGS2 has no contraindications.

PTGS2 is part of a family of prostaglandins which are produced by many enzymes. One is prostaglandin-endoperoxide synthase (PES), which catalyzes the production of prostaglandin H2. The enzymes COX-1 as well as Cox-2 are involved in the production of PGS2. The protein is an important mediator of inflammation, and is targeted by anti-inflammatory medicines.

PTGS2 is an antigen that is monoclonal, created from Human Cox2. It was made by immunizing mice using an artificial peptide which matched the residues around His108 in the human Cox2 protein. This antibody is a powerful way to check COX2 activity in cancer cells as well as other tumors. It is extensively used in clinical trials to stop the growth of tumors.

It is used to identify protaglandins that cause inflammation.

Prostaglandins inflammasome are created in the body by several pathways. One pathway is the cyclooxygenase that produces prostaglandins when arachidonic acids is present. Prostaglandin biosynthesis is increased in tissue that is inflamed and is a factor in the development of the characteristic signs of acute inflammation. Prostaglandins can be produced when there is a mix of mediators of inflammation and the proinflammatory properties of the individual PGs have been established. This review will concentrate on the biosynthesis of and responses to PGs in acute inflammation. Furthermore, the biochemistry as well as the clinical relevance of prostaglandins in atherosclerosis is discussed.

Prostaglandins act as signals for a variety of body processes which include the immune system. The inflammatory response results in the production of high levels of prostaglandins which are associated with redness, swelling, and discomfort. Prostaglandins are part of the body's natural healing process. It has been demonstrated that it can protect against colorectal carcinoma even though there isn't a lot of information about the mechanism.

Researchers have found that PTGS2 is high-yielding in cancer cells in both human and animal models. PTGS2 also regulates inflammation. In addition to detecting inflammation prostaglandins PTGS2 also has significant cardiovascular effects and is a key mediator of edema and pain. It is unclear whether PGI2 is an natural ligand, or an artificial ligand for the PPARg.

Only recently has PTGS2's biochemical significance been revealed. Although it could be linked to NSAID drug pharmacology, it has numerous other functions. This gene is involved in a variety of diseases of inflammation such as arthritis menstrual cramps, and colon cancer. Further research into PTGS2 is necessary to better understand its function in the body's physiology.

There are more than 100 variants of PTGS2 gene. The G>C transition within the promoter region is the most frequent, and it affects the binding of transcription factors. The G>C transformation removes the Sp1 site and creates an E2F binding site. These variants have been known as ss5112606 as well as 899G.

It is a possibility to use it in RT-PCR

The RT-PCR candidate gene PTGS2 is a candidate. Contrary to PTGS1, PTGS2 is expressed in a wide variety of cell types including intestinal epithelia. This gene is crucial because it is a significant marker for cell proliferation. These cells are where the gene is expressed to identify PSC cells. However the process of identifying PSC cells is still an obstacle.

To study PTGS2 expression in human cells the amplification of its three' UTR (UTR) was carried out using primers that target miR-26b. The miR26b sequence was integrated into a luciferase reporter. Cells transfected with the miR-26b sequence displayed marked repression in the activity of luciferase. This decrease in luciferase function was due to a decrease in the levels of PTGS2 mRNA and protein. Additionally, miR-26b blocks the PTGS2 gene in CNE cells.

It is possible to use it in ELISA kit

PTGS2 is a protein that is found in plants and is a popular subject of ELISA tests. A single ELISA kit can determine the presence of PTGS2 or one of the subunits of the protein. Multiple ELISA kits can detect a range of protein levels from a single sample. One ELISA kit can detect a maximum of five million pg/mL within a single experiment.

All of the reagents required for a successful assay are included in ELISA kits. This plate-based test detects and quantifies molecules by using antibodies that are linked to enzymes. Different formats employ different principles for detecting protein concentrations. Picokine(tm), an ELISA kit, uses high-affinity antibodies for detecting native-form proteins. Boster's ELISA kits have been validated by the QC department against immunogenic proteins of relevant superfamilies.