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- Table of Contents
PTGS2 antibody (A00084-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Flow Cytometry analysis of CACO-2 cells using anti-PTGS2 antibody (A00084-2).
Overlay histogram showing CACO-2 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing CACO-2 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing a Western blot for PTGS2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PTGS2 Western Blot GuideBundle Save
Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody
PTGS2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
PTGS2 antibody was positively detected by WB in:
human Hela whole cell, mouse RAW2647(-LPS) whole cell, mouse RAW2647(+LPS) whole cell, human A549- WT whole cell, human A549-PTGS2 KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTGS2 antibody was positively detected by IHC in:
human lung cancer tissue, human pancreatic cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PTGS2 antibody was positively detected by FC in:
CACO-2 cell, HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody
View all PTGS2 Antibodies
View all PTGS2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human COX2/Cyclooxygenase 2/PTGS2 recombinant protein (Position: A18-L604).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
69.0 kDa
Background of PTGS2
Prostaglandin-endoperoxide synthase 2, also known as cyclooxygenase-2 or COX-2, is an enzyme that in humans is encoded by the PTGS2 gene. It is mapped to 1q31.1. Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Cite This Product
Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00084-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
91%
High Homology
Rabbit
90%
High Homology
Cow
89%
Moderate Homology
Sheep
88%
Moderate Homology
Mouse
87%
Moderate Homology
Rat
87%
Moderate Homology
Zebrafish
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PTGS2 antibody
Immunohistochemistry (IHC) protocol for PTGS2 antibody
Flow Cytometry (FC) protocol for PTGS2 antibody
Publications
Application:
Species:
Lycorine Suppresses DPP3-Mediated Nrf2 Signaling, Thereby Reducing SLC7A11 Levels to Trigger Ferroptosis in Colorectal Cancer Cells
Published: 2026-04-19
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 8.30
Species: Human
PMID: 42048794
S-equol promotes ferroptosis in triple negative breast cancer by coordinating NCOA4-mediated ferritinophagy and PPARγ-mediated lipid metabolism
Published: 2026-04-02
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 41925800
In vivo anti-inflammatory, analgesic activities and phytochemical profile of Hu Lisan ethanol extract
Published: 2025-11-03
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 5.40
Species: Rat
PMID: 41192694
Metabolomics reveals glycerophospholipid metabolism as the action mechanism of Jin-Tian-Ge capsules in ameliorating inflammatory pain
Published: 2025-09-08
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB,IHC
Impact Factor: 5.40
Species: Mouse
PMID: 40930294
Molecular characterization of PANoptosis-related genes in chronic kidney disease
Published: 2024-10-28
Journal: PLoS One
Application: IHC
Impact Factor: 2.90
Species: Rat
PMID: 39466748
Anti-inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF-κB and MAPK signaling pathways
Published: 2024-10-25
Journal: FEBS Open Bio
Application: WB
Impact Factor: 2.80
Species: Mouse
PMID: 39455284
Blestriarene C exerts an inhibitory effect on triple-negative breast cancer through multiple signaling pathways
Published: 2024-10-22
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 39502536
Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma
Published: 2024-07-21
Journal: Arab Journal of Gastroenterology
Application: WB
Impact Factor: 1.10
Species: Human
PMID: 39039002
Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study
Published: 2024-06-06
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Mouse
PMID: 38952369
A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
Published: 2024-01-28
Journal: SEPARATION AND PURIFICATION TECHNOLOGY
Application: IHC,WB
Impact Factor: 8.10
Species: Mouse
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties
Published: 2022-08-17
Journal: Foods
Application: WB
Impact Factor: 5.56
Species: Mouse
PMID: 36010488
Old targets, new strategy: Apigenin-7-O-β-d-(-6″-p-coumaroyl)-glucopyranoside prevents endothelial ferroptosis and alleviates intestinal ischemia-reperfusion injury through HO-1 and MAO-B inhibition
Published: 2022-04-07
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 8.10
Species: Human
PMID: 35398494
The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways
Published: 2018-10-29
Journal: Scientific Reports
Application: WB
Impact Factor: 4.12
Species: Human
PMID: 30374107
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Product Promise
We promise Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00084-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026