Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody

Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.

IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2).
PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTGS2 Antibody (A00084-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Effects of maltol on the levels of inflammation cytokines in cisplatin-induced renal toxicity. ( A ) Effects of maltol on the positive expressions of Bax, Bcl-2, iNOS and COX-2 in renal tissues were examined by IHC in renal tissues (magnification × 200), And the column chart shows stained area, semiquantitative analysis of Bax, Bcl-2, iNOS and COX-2 expression in kidneys to IHC. ( B ) Inflammation cytokines level of TNF-α, IL-1β, iNOS and NF-κB in serum of mice were measured by ELISA kits. All values were expressed as mean ± S.D. * p < 0.05, ** p < 0.01 vs . normal group; # p < 0.05, ## p < 0.01 vs . cisplatin group.
Index in PubMed under a CC BY license. PMID: 30374107

Antioxidant and anti-inflammatory effects of OVE in LPS-stimulated RAW264.7 cells. (A, B) Cell viability measured by CCK8 assay. (C) Analysis of ROS levels detected by DCFH-DA probe. (D) Quantitative analysis of gene expression levels of iNos, Il6, Il-1b, Tnfα, and Cox-2 by qRT-PCR. (E) NO production analysis by NO assay. (F) ELISA results of IL6, IL-1β, and TNFα. (G) Protein expression levels of COX-2, IL6, IL-1β, and TNFα. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ####P < 0.0001, compared to the untreated control group; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, compared to the LPS control group.
Index in PubMed under a CC BY license. PMID: 39455284

Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (A00084-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549- WT whole cell lysates,
Lane 2: human A549-PTGS2 KO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (A00084-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.

IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2).
PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTGS2 Antibody (A00084-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Flow Cytometry analysis of CACO-2 cells using anti-PTGS2 antibody (A00084-2).
Overlay histogram showing CACO-2 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HEPA1-6 cells using anti-PTGS2 antibody (A00084-2).
Overlay histogram showing HEPA1-6 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.