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Product Info Summary
| SKU: | EK2289 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | cell lysates. |
Product info
Product Name
Human/Mouse COX-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2289
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human/Mouse COX-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human/Mouse COX-2 in cell lysates. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human/Mouse COX-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2289)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A18-L604
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2289 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2289 is reactive to PTGS2 in Human, Mouse samples
Validated Sample Types
cell lysates.
Application Guarantee
EK2289 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PTGS2
Cyclooxygenase-2 (COX-2), also known as Prostaglandin-endoperoxide synthase 2 (HUGO PTGS2), is an enzyme that in humans is encoded by the PTGS2 gene. PTGS2 (prostaglandin-endoperoxide synthase 2) is a gene that encodes an enzyme responsible for catalyzing the conversion of arachidonic acid into prostaglandins. Prostaglandins are lipid signaling molecules that play crucial roles in regulating inflammation, pain, and reproductive processes. PTGS2 is expressed in various tissues, particularly in sites of inflammation and certain tumor cells, where its expression levels are often significantly elevated. The activity of this gene is regulated by various stimuli, including cytokines, hormones, and growth factors, making it a key player in pathological conditions such as inflammation, cancer, and cardiovascular diseases. Due to its involvement in these diseases, PTGS2 has become a potential drug target, especially in the development of nonsteroidal anti-inflammatory drugs (NSAIDs).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2289-CAP | Anti-Human, Mouse PTGS2 Pre-coated 96-well strip microplate | 1 |
| EK2289-ST | Human, Mouse PTGS2 Standard | 2 vials, 10 ng/tube |
| EK2289-DA | Human, Mouse PTGS2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.053 | 0.13 | 0.203 | 0.331 | 0.591 | 1.08 | 1.738 | 2.604 |
Data Example Images
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Human/Mouse COX-2 ELISA Kit PicoKine standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PTGS2, Dilution ratio of 1:1..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human/Mouse COX-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 272 | 936 | 2278 | 187 | 639 | 4978 |
| Standard deviation | 9.36 | 41.56 | 185.01 | 4.64 | 45.50 | 173.73 |
| CV(%) | 3.4% | 4.4% | 6.7% | 2.5% | 7.1% | 3.5% |
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