PTPRC Antibodies

Receptor-type tyrosine-protein phosphatase C (PTPRC)

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4.

The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the initial one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Gene Information

Human
Gene Name:	PTPRC
Uniprot:	P08575
Entrez:	5788

Family

Belongs to:
protein-tyrosine phosphatase family

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

Molecular Weight

Mass (kDA):
147.486 kDA

Genomic Context

Human
Location:	1q31.3-q32.1
Sequence:	1; NC_000001.11 (198638968..198757476)

Tissue Specificity

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts.

Diseases

• Neoplasms
• Inflammation
• Malignant Neoplasms
• Tissue Adhesions
• Autoimmune Reaction
• Leukemia
• Fibrosis
• Carcinoma
• Pathologic Neovascularization
• Multiple Sclerosis
• Sclerosis
• Lymphoma
• Tumor Angiogenesis

• Autoimmune Diseases
• Immunologic Deficiency Syndromes
• Hiv Infections
• Autoimmunity
• Arthritis
• Rheumatoid Arthritis
• Neoplasm Metastasis

Interacting Proteins

• CSK
• CTNNB1
• ERBB2
• ITGAL
• LCK

• Lck
• LGALS1
• TEK

Pathways

• Pathogenesis
• Secretion
• Angiogenesis
• Regeneration
• Localization
• Cell Adhesion
• Cell Activation
• Immune Response
• Cell Proliferation
• T Cell Activation
• Cell Differentiation
• Cell Cycle
• Cell Development

• Inflammatory Response
• Cytokine Secretion
• Cytokine Production
• Cell Death
• Glycosylation
• Phagocytosis
• Cell Migration

PTMs

• Phosphorylation
• Dephosphorylation
• Cleavage
• Reduction

• Glycosylation
• Acetylation
• Methylation
• Ubiquitination

Research Areas

• Mesenchymal Stem Cell Markers
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PTPRC

Best Uses Of The PTPRC Marker By Boster Bio

Boster Bio's Anti CD45RB (B Cell Marker) PTPRC Monoclonal Antibody is the best option if you are looking for a monoclonal antibody that reacts with Human cells. It is non-hazardous and may be purchased with or without BSA. If you are concerned about the safety or effectiveness of the antibody, you could purchase an antibody that blocks to it. This peptide can be bought in the 1.0mg/ml range for a cost that is based on the length of the immunogen. Boster Bio has validated its antibodies by testing them on positive and negative samples.

ELISA

The ELISA employing the PTPRC marker made by Boster Bio is an extremely sensitive and precise method for the quantitative measurement of PTPRC. The marker is part of the family of proteins tyrosine-phosphatases that regulates numerous cell processes. The PTPRC marker has an extracellular domain, a transmembrane segment and two tandem intracytoplasmic catalytic domains. This glycoprotein is found on the cell's surface and lymphocytes. It is a major regulator for signaling by B-cell and T-cell antigen receptors. It also regulates Src-family Kinases.

ELISA kits include all essential reagents needed and employ a variety of techniques. Different formats utilize a similar principle to bind antibodies to biomolecules. Boster's PTPRC ELISA Kit uses high-affinity antibodies to detect native-form proteins. Boster confirms their ELISA kits against proteins belonging to the same superfamily, and also similar immunogenic proteins.

For an PTPRC ELISA, Boster Bio offers an anti-PTPRC antibody for Human, Mouse, and Rat. It is part of the Picoband(tm), and has been employed in ELISA as well as WB applications. The PTPRC antibody is reconstituted at 4 degC. Boster Bio offers a low-cost and easy method for identifying cell types and their activity.

Immunofluorescence

The PTPRC marker is a fluorescent protein which is found on the surface of human cells. This marker is extremely specific and has excellent immunofluorescence capabilities. The PTPRC marker is available in the form of a monoclonal antibody or an avidin-biotin combination. Boster Bio uses a proprietary ELISA platform known as PicoKine(tm). This platform provides high-sensitivity ELISA kits that can be used in a variety applications.

The anti-Desmin antibody from Boster Bio reacts with Human, Rabbit, and Rat cells. It can be stored at -20°C for up to 6 months and is stable at 4degC over a month. Monoclonal antibodies are available and can be conjugated with FITC. They react with a rabbit or human isotype antibody, as well as mouse ascites fluid.

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References

PMID: 2824653 by Streuli M., et al. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.

PMID: 2956090 by Ralph S.J., et al. Structural variants of human T200 glycoprotein (leukocyte-common antigen).