Anti-Ms CD45 Purified Ptprc Monoclonal Antibody

Product Name

Anti-Ms CD45 Purified Ptprc Monoclonal Antibody

View all Ptprc Antibodies

SKU/Catalog Number

M00555-4

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD45 Purified Ptprc Monoclonal Antibody (Catalog# M00555-4). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD45 Purified Ptprc Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555-4)

Host

Rat

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-05

Isotype

Rat IgG

Immunogen

Murine peripheral blood leukocytes. The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.

Reactive Species

M00555-4 is reactive to Ptprc in Mouse

Observed Molecular Weight

19 kDa

Background of Ptprc

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00555-4 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2 μg/ml; positive control: murine peripheral blood leukocytes.

Validation Images & Assay Conditions

See full target information Ptprc

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