Anti-CD45 PTPRC Antibody

Boster Bio Anti-CD45 PTPRC Antibody catalog # A00555. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00555
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD45 PTPRC Antibody

See all PTPRC products

SKU/Catalog Number

A00555

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CD45 PTPRC Antibody catalog # A00555. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD45 PTPRC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00555)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD45 around the non-phosphorylation site of S1007.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00555 is reactive to PTPRC in Human, Mouse, Rat

Applications

A00555 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For PTPRC (Source: Uniprot.Org, NCBI)

Uniprot ID

P08575

Gene ID

5788

Gene Name

PTPRC

Full Name

Receptor-type tyrosine-protein phosphatase C

Weight

147.486kDa

Superfamily

protein-tyrosine phosphatase family

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PTPRC, check out the PTPRC Infographic

PTPRC infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PTPRC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00555 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

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15 Customer Q&As for Anti-CD45 PTPRC Antibody

Question

My boss were content with the WB result of your anti-CD45 antibody. However we have been able to see positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PTPRC supposed to be expressed?

P. Parker

Verified customer

Asked: 2020-04-06

Answer

From what I have seen in literature, leukemic t-cell does express PTPRC. Generally PTPRC expresses in cell membrane. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-04-06

Question

Can you help my question with product A00555, anti-CD45 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00555 anti-CD45 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-03

Question

Is this A00555 anti-CD45 antibody reactive to the isotypes of PTPRC?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

The immunogen of A00555 anti-CD45 antibody is Synthesized peptide derived from human CD45 around the non-phosphorylation site of S1007. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-16

Question

Will anti-CD45 antibody A00555 work for WB with liver?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

According to the expression profile of liver, PTPRC is highly expressed in liver. So, it is likely that anti-CD45 antibody A00555 will work for WB with liver.

Boster Scientific Support

Answered: 2019-08-22

Question

I am interested in using your anti-CD45 antibody for regulation of phagocytosis studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

Thank you for your inquiry. This A00555 anti-CD45 antibody is tested on jurkat cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-12

Question

We are interested in to test anti-CD45 antibody A00555 on human liver for research purposes, then I may be interested in using anti-CD45 antibody A00555 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Parker

Verified customer

Asked: 2019-05-28

Answer

The products we sell, including anti-CD45 antibody A00555, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-28

Question

I have attached the WB image, lot number and protocol we used for liver using anti-CD45 antibody A00555. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-02-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-28

Question

We are currently using anti-CD45 antibody A00555 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

J. Gonzalez

Verified customer

Asked: 2018-10-19

Answer

The anti-CD45 antibody (A00555) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-19

Question

Is there a BSA free version of anti-CD45 antibody A00555 available?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD45 antibody A00555 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-03

Question

I see that the anti-CD45 antibody A00555 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-02-06

Question

I was wanting to use your anti-CD45 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2017-07-11

Answer

You can see on the product datasheet, A00555 anti-CD45 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-11

Question

Would A00555 anti-CD45 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Kulkarni

Verified customer

Asked: 2016-01-01

Answer

It shows on the product datasheet, A00555 anti-CD45 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-01-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD45 antibody A00555. Let me know if you need anything else.

P. Li

Verified customer

Asked: 2015-01-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-01-20

Question

Is a blocking peptide available for product anti-CD45 antibody (A00555)?

N. Jones

Verified customer

Asked: 2014-12-08

Answer

We do provide the blocking peptide for product anti-CD45 antibody (A00555). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-12-08

Question

We have seen staining in mouse placenta. Any tips? Is anti-CD45 antibody supposed to stain placenta positively?

P. Evans

Verified customer

Asked: 2014-12-04

Answer

From what I have seen in literature placenta does express PTPRC. From what I have seen in Uniprot.org, PTPRC is expressed in blood, lymphocyte, synovium, placenta, t-cell, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2014-12-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00555
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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