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Product Info Summary
| SKU: | M00555-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Hu CD45 Purified PTPRC Monoclonal Antibody
SKU/Catalog Number
M00555-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Catalog# M00555-3). Tested in Flow Cytometry, IP, WB, IHC-P, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-28
Isotype
Mouse IgG1
Immunogen
Human thymocytes and T lymphocytes. The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Reactive Species
M00555-3 is reactive to PTPRC in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
147.5 kDa
Background of PTPRC
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00555-3 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used.
Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
Flow cytometry: Recommended dilution: 1 μg/ml.
Validation Images & Assay Conditions
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Separation of human lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) purified antibody (concentration in sample 2 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) purified antibody (concentration in sample 2 µg/ml) GAM APC.
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Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28, green). DNA visualized by DAPI (blue)
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Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28), 10 µg/ml. Commercially tested by LifeSpan BioSciences.
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Anti-Hu CD45 Purified (clone MEM-28) works in WB application under non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-28 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.
CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
Specific Publications For Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3)
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