SMAD5 Antibodies

Mothers against decapentaplegic homolog 5 (SMAD5)

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

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Gene Information

Human
Gene Name:	SMAD5
Uniprot:	Q99717
Entrez:	4090

Family

Belongs to:
dwarfin/SMAD family

Alternative Names

Dwfc; hSmad5; JV5-1; JV5-1DKFZp781O1323; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila); MADH5; MADH5mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5DKFZp781C1895; SMAD, mothers against DPP homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5; Smad5

Molecular Weight

Mass (kDA):
52.258 kDA

Genomic Context

Human
Location:	5q31.1
Sequence:	5; NC_000005.10 (136132845..136182734)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Diseases

• Neoplasms
• Malignant Neoplasms
• Tumor Angiogenesis
• Carcinoma
• Leukemia
• Myeloid Leukemia
• Malignant Paraganglionic Neoplasm
• Hypertensive Disease
• Cell Transformation, Neoplastic
• Tissue Adhesions
• Idiopathic Pulmonary Hypertension
• Pulmonary Hypertension
• Haploinsufficiency

• Kidney Diseases
• Neoplasm Metastasis
• Anemia
• Diabetes Mellitus
• Diabetic Nephropathy
• Subacute Bacterial Endocarditis
• Infertility

Interacting Proteins

• DDX5

Pathways

• Cell Proliferation
• Osteoblast Differentiation
• Cell Differentiation
• Angiogenesis
• Gastrulation
• Pathogenesis
• Localization
• Bmp Signaling Pathway
• Cell Cycle
• Secretion
• Ossification
• Cell Growth
• Vasculogenesis

• Cell Migration
• Regeneration
• Reverse Transcription
• Cell Death
• Translation
• Bone Development
• Rna Interference

PTMs

• Phosphorylation
• Ubiquitination
• Dephosphorylation

• Cleavage
• Methylation
• Ubiquitinylation

• Acetylation

Research Areas

• Phospho-Specific
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMAD5

Best Uses Of The SMAD5 Marker

You've come to a good place if looking for a biomarker or a way to test your samples. SMAD5 comes from the TGFbeta group of proteins that inhibit the progression and progression of PF, EMT. This biomarker could help you reach your goals. Boster Bio stocks this biomarker. This boster marker works by blocking genes involved in mesenchymal and mesenchymal differentiation. This marker is a great choice. Boster Bio has many applications.

SMAD5 is a member of the TGFbeta protein family

Boster Bio AntiSMAD5 antibody has been tested for IHC and WB in human and mouse cells. It reacts with the SMAD5 antibody in human and mouse cells. This makes it a powerful and versatile immunoreactive instrument. There are many formats of the Boster antibody available, including WB (IHC), and Immunofluorescence.

The miR-122-5p has been shown to negatively regulate Smad5 in various cell types. It inhibits Smad5's transcriptional activity and has therapeutic implications. The miR-122-5p mimic inhibits the activity of Smad5 and prevents a-SMA, COL-1, and vimentin from expressing in cells. This means that miR-122-5p is a candidate for PF tumors.

It is a mesenchymal indicator

SMAD5 can be upregulated by miR195, which is a mesendichmal indicator. The increased expressions of SMAD5–AS1 as well as miR–195 in different cell lines can be attributed to the upregulations in SMAD5–AS1 or miR–195. In addition, miR-195 and SMAD5 compete for binding sites with miR-195.

We used a RIP kit to detect SMAD5-AS1 protein binding to AGO2 protein in CNE-2 cells. Sections were fixed in 10% formalin. Then, sections were incubated for 24 hours with a rabbit monoclonal antibodies to SMAD5 or goat anti-rabbit IgG. SMAD5 - AS1 magnetic beads coated with 5 mg rabbit anti-human GO2 antibody were incubated. 100 mL Cell Lysate was then added to the magnetic bead. To collect the protein-bead compounds, magnetic pedestals were used. Images were captured with Image J software.

The results of this study indicated that SMAD5 has been found to be upregulated in CNE-2-cells and inhibits cell migration, invasion, proliferation. The siRNA mimicked miR195 while SMAD5 inhibited BMP2-induced proliferation, migration, apoptosis, and cell proliferation. These results are consistent with previous studies. We conclude that SMAD5 in Boster Bio is a mesenchymal marker.

It stops EMT progress and blocks PF progression

The SMAD5 gene is involved in regulating cell growth and proliferation. It regulates the activity a number RTKs, which results in the formation focal adhesions as well as directional migration. It prevents apoptosis. This condition can lead to various diseases, such as cancer. It is not yet clear if the SMAD5 gene plays a protective or prognostic role in cancer progression.

By competitively binding to miR195, the SMAD5–AS1 increases SMAD5 expression within NPC cells. The inhibition of NPC cell growth through EMT is caused by the downregulation of SMAD5–AS1. This suggests that it may be a therapeutic target in NPC intervention. This finding could have important implications for the treatment PF as well other cancers. Future research will be done.

We examined cellular phenotypes of PF mice. We identified a number of genes that have similar functions using miR target gene prediction software. This group identified many lncRNAs. Then, they analyzed their activities. The SMAD5 antisense RNA (SMAD5-AS1) and the microRNA-195, which is known to bind to SMAD5. We then used these results to determine if the SMAD5 molecule in inhibited PF/EMT in NPC-cells.

Boster Bio can supply it

This antibody detects SMAD5. Boster stocks primary antibodies that were validated by multiple platforms. This ensures high affinity as well as high specificity. You can also get free antibody kits to help you with your research. Boster's high affinity SMAD5 Marker will allow you to get the best out of their antibody selection. Here are more details about Boster’s antibody.

SMAD5 is a receptor for the kinase AKT enzyme kinase. The body's reaction to SMAD5 triggers a inflammatory response. It has a direct influence on the development inflammation. Boster Bio provides a monoclonal immunoglobulin to detect this protein. This antibody reacts with Human antigen CD105. It can be measured by Western Blot.

It is reliable

SMAD5 is a protein involved in the transforming growth factor beta signaling pathway. This pathway inhibits the growth of hematopoietic protocells. SMAD5 activation is caused by bone morphogenetic types 1 receptor kinase. Due to alternative gene splicing, it is composed of multiple variants. It is useful in diagnosing disease and determining treatment strategies because SMAD5 is involved with multiple biological processes.

Using immunohistochemistry, we detected the SMAD5 protein expression in NPC tissues. We also determined the expressions of miR195 as well as SMAD5 using western blot analyses. The SMAD5 gene was expressed in NPC tissue, but not in nearby normal tissues. However, miR195 expression was downregulated. This study demonstrates that the SMAD5 indicator is a reliable biomarker for NPC.

It is a primary antigen

Boster Bio's SMAD5 antibody is an immunogen which reacts to human cells. This antibody is non-hazardous and has been tested in both WB and ELISA applications. It is designed to recognize the sequence of human SMAD1/2/3. A blocking peptide can be purchased at 1.0 mg/ml. It is recommended that you use the antibody together with a blocking progecide to further reduce cross-reactivity.

Boster Bio SMAD5 Marker is designed to enable researchers to examine SMAD5 activity in human cells. It is available in two forms: a primary and secondary antibody. The primary antibody binds antigens within the body. Secondary antibodies can be combined with different antigens to allow researchers to have more information about their specimens.

It is a reagent

MiR-122-5p is responsible for controlling the SMAD5 molecule. This molecule is directly associated with the 3'-UTR of Smad5. Inhibition of Smad5 expression by PF was shown to block PF and EMT progression. The results suggest that miR-122-5p is a promising reagent for PF research. Two versions of the Boster BioSMAD5 marker are available. Both reagents have the ability to be used with microarray and fluorescent devices.

The SMAD5 molecule is a molecule that binds miR-125-2p, a negative transcriptional regulator of Smad5. It also promotes Wnt10b (SMAD5) and miR-122-5p. SMAD5 plays a critical role in Wnt signaling and is found in many cell types. Researchers can measure the activity and expression of Smad5 in real-time by detecting it in cells.