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Product Info Summary
| SKU: | M01423-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC, WB |
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Product info
Product Name
Anti-Smad5 (C-terminus) monoclonal Antibody
SKU/Catalog Number
M01423-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Smad5 (C-terminus) monoclonal Antibody catalog # M01423-1. Tested in WB,ICC,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad5 (C-terminus) monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01423-1)
Host
Mouse
Contents
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Monoclonal
Clone Number
Q32
Isotype
IgG1
Immunogen
Synthetic peptide, corresponding to the C-terminus of Human Smad5.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01423-1 is reactive to SMAD5 in Human, Mouse
Calculated molecular weight
52.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01423-1 is guaranteed for Flow Cytometry, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:50-100
FC: 1:50-100
Validation Images & Assay Conditions
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Western blot detection of SMAD5 antibody in Mouse testis cell lysates using SMAD5 antibody (1:1000 diluted) .
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Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
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Immunocytochemistry of HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:75.
Specific Publications For Anti-Smad5 (C-terminus) monoclonal Antibody (M01423-1)
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