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- Table of Contents
SMAD5 antibody (P01423-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody.
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Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody
SMAD5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SMAD5 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SMAD5 antibody was positively detected by IHC in:
human pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody
View all SMAD5 Antibodies
View all SMAD5 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Smad5 (S463/465)
Observed Molecular Weight
52 kDa
Calculated Molecular Weight
52.3 kDa
Cite This Product
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01423-1)
Protocols
Western Blot protocol for SMAD5 antibody
Immunohistochemistry (IHC) protocol for SMAD5 antibody
Publications
Application:
Species:
Pilose antler extract promotes hair growth in androgenic alopecia mice by promoting the initial anagen phase
Published: 2024-04-01
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB,IF
Impact Factor: 6.90
Species: Mouse
PMID: 38565060
ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-β1/Smad Pathway Following Experimental Traumatic Brain Injury
Published: 2023-06-27
Journal: JOURNAL OF NEUROTRAUMA
Application: IF
Impact Factor: 4.20
Species: Rat,Mouse
PMID: 37029898
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Submit A ReviewProduct Promise
We promise Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P01423-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 17, 2026