Anti-SMAD5 Antibody

Product Name

Anti-SMAD5 Antibody

View all SMAD5 Antibodies

SKU/Catalog Number

PA2115

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMAD5 Antibody catalog # PA2115. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMAD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2115)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2115 is reactive to SMAD5 in Human, Mouse, Rat

Applications

PA2115 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

52.258kDa

Background of SMAD5

Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Rat Intestine Tissue

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Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-SMAD5 antibody, PA2115, Western blotting
All lanes: Anti SMAD5 (PA2115) at 0.5ug/ml
Lane 1: K562 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: PC 1-2 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 52 KD
Observed bind size: 52KD

Gene/Protein Information For SMAD5 (Source: Uniprot.org, NCBI)

Alternative Names

Dwfc; hSmad5; JV5-1; JV5-1DKFZp781O1323; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila); MADH5; MADH5mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5DKFZp781C1895; SMAD, mothers against DPP homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5; Smad5 SMAD5 DWFC, JV5-1, MADH5 SMAD family member 5 mothers against decapentaplegic homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5

For more info on SMAD5, check out the SMAD5 Infographic

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In this infographic, you will see the following information for SMAD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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