Anti-SMAD5 Antibody Picoband®

Product Name

Anti-SMAD5 Antibody Picoband®

View all SMAD5 Antibodies

SKU/Catalog Number

PA2115
BA3079 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMAD5 Antibody catalog # PA2115. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMAD5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2115)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2115 is reactive to SMAD5 in Human, Mouse, Rat

Observed Molecular Weight

52 kDa

Calculated molecular weight

52.3 kDa

Background of SMAD5

Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2115 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: K562 Whole Cell, JURKAT Whole Cell, PC 1-2 Whole Cell, HELA Whole Cell, SMMC Whole Cell
IHC: Rat Intestine Tissue, Human Intestinal Cancer Tissue, Human Lung Cancer Tissue

Validation Images & Assay Conditions

See full target information SMAD5

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