Product Info Summary
SKU: | PA2115 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-SMAD5 Antibody
SKU/Catalog Number
PA2115
BA3079 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMAD5 Antibody catalog # PA2115. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMAD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2115)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2115 is reactive to SMAD5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
52258 MW
Background of SMAD5
Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2115 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: K562 Whole Cell, JURKAT Whole Cell, PC 1-2 Whole Cell, HELA Whole Cell, SMMC Whole Cell
IHC: Rat Intestine Tissue, Human Intestinal Cancer Tissue, Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-SMAD5 antibody, PA2115, Western blotting
All lanes: Anti SMAD5 (PA2115) at 0.5ug/ml
Lane 1: K562 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: PC 1-2 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 52 KD
Observed bind size: 52KD
Protein Target Info & Infographic
Gene/Protein Information For SMAD5 (Source: Uniprot.org, NCBI)
Gene Name
SMAD5
Full Name
Mothers against decapentaplegic homolog 5
Weight
52258 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5; SMAD5 DWFC, JV5-1, MADH5 SMAD family member 5 mothers against decapentaplegic homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD5, check out the SMAD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMAD5 Antibody (PA2115)
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No publications found for PA2115
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1 Customer Q&As for Anti-SMAD5 Antibody
Question
We are currently using anti-SMAD5 antibody PA2115 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
The anti-SMAD5 antibody (PA2115) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-20