This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SMAD5 antibody (PA2115) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Anti-SMAD5 antibody, PA2115, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Designing a Western blot or IHC for SMAD5? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
Bundle Save
Anti-SMAD5 Antibody Picoband®
SMAD5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
SMAD5 antibody was positively detected by WB in:
K562 Whole Cell, JURKAT Whole Cell, PC 1-2 Whole Cell, HELA Whole Cell, SMMC Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SMAD5 antibody was positively detected by IHC in:
Rat Intestine Tissue, Human Intestinal Cancer Tissue, Human Lung Cancer Tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
Rat
Tested
Recommend
Human
Tested
Recommend
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Mouse
Predicted
Predicted
Rabbit
Predicted
Predicted
Product Information
Product Name
Anti-SMAD5 Antibody Picoband®
View all SMAD5 Antibodies
View all SMAD5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated Molecular Weight
52.3 kDa
Background of SMAD5
Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.
Cite This Product
Anti-SMAD5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2115)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
95%
High Homology
Rabbit
95%
High Homology
Zebrafish
55%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SMAD5 antibody
Immunohistochemistry (IHC) protocol for SMAD5 antibody
Review
Have you used Anti-SMAD5 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-SMAD5 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA2115
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026