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Product Info Summary
| SKU: | M01423 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Smad5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01423
BM4097 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Smad5 Rabbit Monoclonal Antibody catalog # M01423. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01423)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BDB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad5
Reactive Species
M01423 is reactive to SMAD5 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01423 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Caco-2 whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse Hepa1-6 whole cell, mouse NIH/3T3 whole cell
IHC: Human stomach cancer tissue, Human Hodgkin's lymphoma tissue, Human glioblastoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Smad5 using anti-Smad5 antibody (M01423).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 7: mouse Hepa1-6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smad5 antigen affinity purified monoclonal antibody (Catalog # M01423) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smad5 at approximately 52 kDa. The expected band size for Smad5 is at 52 kDa.
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Immunohistochemical analysis of paraffin-embedded Human stomach cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:50 dilution.
Specific Publications For Anti-Smad5 Rabbit Monoclonal Antibody (M01423)
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Customer Q&As
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5 Customer Q&As for Anti-Smad5 Rabbit Monoclonal Antibody
Question
I am looking for to test anti-Smad5 Rabbit Monoclonal antibody M01423 on human ovarian carcinoma for research purposes, then I may be interested in using anti-Smad5 Rabbit Monoclonal antibody M01423 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The products we sell, including anti-Smad5 Rabbit Monoclonal antibody M01423, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-21
Question
See attached the WB image, lot number and protocol we used for ovarian carcinoma using anti-Smad5 Rabbit Monoclonal antibody M01423. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Does M01423 anti-Smad5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
You can see on the product datasheet, M01423 anti-Smad5 Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-05
Question
Would anti-Smad5 Rabbit Monoclonal antibody M01423 work on feline IHC with female gonad?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
Our lab technicians have not tested anti-Smad5 Rabbit Monoclonal antibody M01423 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Smad5 Rabbit Monoclonal antibody M01423 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline female gonad in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-06
Question
Is a blocking peptide available for product anti-Smad5 Rabbit Monoclonal antibody (M01423)?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
We do provide the blocking peptide for product anti-Smad5 Rabbit Monoclonal antibody (M01423). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-09
