BRSK1 (NM_032430) Human Over-expression Lysate

Lysate of BRSK1 over-expression cell lines, WB control

Transient overexpression lysate of BR serine/threonine kinase 1 (BRSK1)

Product Info Summary

SKU: LS084446
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of BR serine/threonine kinase 1 (BRSK1)

Product Name

BRSK1 (NM_032430) Human Over-expression Lysate

See all BRSK1 products

SKU/Catalog Number

LS084446

Size

100 µg

Description

Transient overexpression lysate of BR serine/threonine kinase 1 (BRSK1)

Storage & Handling

Cite This Product

BRSK1 (NM_032430) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084446)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for BRSK1 (Source: Uniprot.org, NCBI)

Gene Name

BRSK1

Full Name

Serine/threonine-protein kinase BRSK1

Weight

85.087kDa

Superfamily

protein kinase superfamily

Alternative Names

BR serine/threonine kinase 1; EC 2.7.11; EC 2.7.11.1; FLJ43009; KIAA1811BR serine/threonine-protein kinase 1; protein kinase SAD1A; SAD1 kinase; SAD1; Serine/threonine-protein kinase SAD-B BRSK1 hSAD1 BR serine/threonine kinase 1 serine/threonine-protein kinase BRSK1|BR serine/threonine-protein kinase 1|SAD1 homolog|SAD1 kinase|brain-selective kinase 1|brain-specific serine/threonine-protein kinase 1|protein kinase SAD1A|serine/threonine-protein kinase SAD-B|synapses of Amphids Defective homolog 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on BRSK1, check out the BRSK1 Infographic

BRSK1 infographic

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In this infographic, you will see the following information for BRSK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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