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Product Info Summary
|Description:||Transient overexpression lysate of BR serine/threonine kinase 1 (BRSK1)|
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BRSK1 (NM_032430) Human Over-expression Lysate
Transient overexpression lysate of BR serine/threonine kinase 1 (BRSK1)
Storage & Handling
Cite This Product
BRSK1 (NM_032430) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084446)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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BRSK1 Human Over-expression Lysates NM_032430.
Western blot validation of overexpression lysate (LS084446) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BRSK1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for BRSK1 (Source: Uniprot.org, NCBI)
Serine/threonine-protein kinase BRSK1
protein kinase superfamily
BR serine/threonine kinase 1; EC 2.7.11; EC 220.127.116.11; FLJ43009; KIAA1811BR serine/threonine-protein kinase 1; protein kinase SAD1A; SAD1 kinase; SAD1; Serine/threonine-protein kinase SAD-B BRSK1 hSAD1 BR serine/threonine kinase 1 serine/threonine-protein kinase BRSK1|BR serine/threonine-protein kinase 1|SAD1 homolog|SAD1 kinase|brain-selective kinase 1|brain-specific serine/threonine-protein kinase 1|protein kinase SAD1A|serine/threonine-protein kinase SAD-B|synapses of Amphids Defective homolog 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS084446
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