KIR2DL1 (NM_014218) Human Over-expression Lysate

Product Name

KIR2DL1 (NM_014218) Human Over-expression Lysate

See all KIR2DL1 products

SKU/Catalog Number

LS003802

Size

100 µg

Description

Transient overexpression lysate of killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1 (KIR2DL1)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

KIR2DL1 (NM_014218) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003802)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for KIR2DL1 (Source: Uniprot.org, NCBI)

Alternative Names

CD158a; KIR2DL1/KIR2DS5 KIR2DL1 CD158A, KIR-K64, KIR221, KIR2DL3, NKAT, NKAT-1, NKAT1, p58.1 killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1 killer cell immunoglobulin-like receptor 2DL1|CD158 -like family member A|KIR2DL protein|KIR2DL1/3DL2|MHC class I NK cell receptor|killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1|killer inhibitory receptor 2-2-1|natural killer-associated transcript 1|p58 NK cell inhibitory receptor NKR-K6|p58 NK receptor CL-42/47.11|p58 killer cell inhibitory receptor KIR-K64|p58 natural killer cell receptor clones CL-42/47.11|p58.1 MHC class-I-specific NK receptor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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