Click image to see more details
Product Info Summary
| SKU: | M04201-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16)
SKU/Catalog Number
M04201-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16) catalog # M04201-1. Tested in WB, IHC applications. This antibody reacts with Human, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04201-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
33I16
Immunogen
Recombinant protein within human Integrin beta 5 aa 3-717.
Reactive Species
M04201-1 is reactive to ITGB5 in Human, Monkey, Rat
Calculated molecular weight
88.1 kDa
Background of ITGB5
This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04201-1 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 1:500-2000 | |
| Immunohistochemistry (IHC) | 1:50-200 |
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Integrin Beta 5/ITGB5 using anti-Integrin Beta 5/ITGB5 antibody (M04201-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 5/ITGB5 antigen affinity purified monoclonal antibody (M04201-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Integrin Beta 5/ITGB5 at approximately 88 kDa. The expected band size for Integrin Beta 5/ITGB5 is at 88 kDa.
Specific Publications For Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16) (M04201-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Integrin Beta 5/ITGB5 Antibody (Monoclonal, 33I16)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
