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Product Info Summary
| SKU: | M02949 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ChIP, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KAT6A Antibody (Monoclonal, 33K13)
SKU/Catalog Number
M02949
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-KAT6A Antibody (Monoclonal, 33K13) catalog # M02949. Tested in WB, IHC, ICC/IF, Flow Cytometry, ChIP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT6A Antibody (Monoclonal, 33K13) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02949)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
33K13
Immunogen
Recombinant protein within human KAT6A aa 787-1032.
Reactive Species
M02949 is reactive to KAT6A in Human, Mouse, Rat
Calculated molecular weight
225.0 kDa
Background of KAT6A
This gene encodes a member of the MOZ, YBFR2, SAS2, TIP60 family of histone acetyltransferases. The protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain. It is part of a complex that acetylates lysine-9 residues in histone 3, and in addition, it acts as a co-activator for several transcription factors. Allelic variants of this gene are associated with an autosomal dominant form of cognitive disability. Chromosomal translocations of this gene are associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02949 is guaranteed for ChIP, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 1:500-2000 | |
| Immunohistochemistry (IHC) | 1:50-200 | |
| Immunofluorescence (IF) | 1:50-200 | |
| Flow Cytometry (FC) | 1:50-200 |
Validation Images & Assay Conditions
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IHC analysis of KAT6A using anti-KAT6A antibody (M02949).
KAT6A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT6A Antibody (M02949) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of KAT6A using anti-KAT6A antibody (M02949).
KAT6A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT6A Antibody (M02949) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-KAT6A Antibody (Monoclonal, 33K13) (M02949)
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