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Product Info Summary
| SKU: | M02001 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Prosurfactant Protein C/SFTPC Antibody (Monoclonal, 34S34)
SKU/Catalog Number
M02001
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Prosurfactant Protein C/SFTPC Antibody (Monoclonal, 34S34) catalog # M02001. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prosurfactant Protein C/SFTPC Antibody (Monoclonal, 34S34) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02001)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34S34
Immunogen
Synthetic peptide within human Prosurfactant Protein C aa 3-48.
Reactive Species
M02001 is reactive to SFTPC in Human, Mouse, Rat
Calculated molecular weight
21.1 kDa
Background of SFTPC
SFTPC(Surfactant pulmonary -associated protein C), also known as SFTP2, SPC, PSP-C or PULMONARY SURFACTANT PROTEIN SP5, is a protein that in humans is encoded by the SFTPC gene. It is a membrane protein which manufactures surfactant. Glasser et al.(1988) assigned the SFTPC gene, symbolized SFTP2 by the Human Gene Mapping nomenclature committee, to chromosome 8 by hybridization to somatic cell hybrid DNA. Wood et al.(1994) identified 4 cosmids at the SFTP2 locus by cDNA hybridization. Using a polymorphic CA repeat in the SFTP2 gene and linkage analysis in 8 CEPH families, they located the gene close to D8S298; maximum lod = 19.3 at theta = 0.01. They regionalized the gene to 8p21 by fluorescence in situ hybridization. Moore et al.(1992) showed that the corresponding mouse gene, Sftp2, maps to chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02001 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 1:500-2000 | |
| Immunohistochemistry (IHC) | 1:50-200 | |
| Immunofluorescence (IF) | 1:50-200 | |
| Flow Cytometry (FC) | 1:50-200 |
Validation Images & Assay Conditions
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Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (M02001).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified monoclonal antibody (M02001) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21 kDa. The expected band size for Prosurfactant Protein C/SFTPC is at 21 kDa.
Specific Publications For Anti-Prosurfactant Protein C/SFTPC Antibody (Monoclonal, 34S34) (M02001)
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