Anti-SPRYD2 CMYA5 Antibody

Product Name

Anti-SPRYD2 CMYA5 Antibody

View all CMYA5 Antibodies

SKU/Catalog Number

A08262

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SPRYD2 CMYA5 Antibody (Catalog # A08262). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SPRYD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SPRYD2 CMYA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08262)

Host

Rabbit

Contents

SPRYD2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2. The immunogen is located within amino acids 3810 - 3860 of SPRYD2.

Cross-reactivity

SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.

Reactive Species

A08262 is reactive to CMYA5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

449.2 kDa

Background of CMYA5

SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08262 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Recommend Dilution

SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CMYA5

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