Product Info Summary
SKU: | M09029 |
---|---|
Size: | 100 µg |
Reactive Species: | Human |
Host: | Mouse |
Application: | WB, FACS |
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Product info
Product Name
Anti-APIP Antibody (Monoclonal, ABM19C7)
SKU/Catalog Number
M09029
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-APIP Antibody (Monoclonal, ABM19C7) (Catalog# M09029). Tested in WB, FACS application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-APIP Antibody (Monoclonal, ABM19C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09029)
Host
Mouse
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Monoclonal
Clone Number
ABM19C7
Isotype
IgG2b, Kappa
Immunogen
Full length recombinant protein of APIP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M09029 is reactive to APIP in Human
Applications
M09029 is guaranteed for WB, FACS Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.125kDa
Background of APIP
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 4-6 µg/ml
FACS: 0.5-1 µg/10^6
Validation Images & Assay Conditions
Click image to see more details
Intracellular flow cytometric analysis of APIP in HeLa cells using 0.5 &mu
Click image to see more details
Western blot analysis of APIP. Anti-APIP antibody (Clone: ABM19C7) was used at 4 &mu
Protein Target Info & Infographic
Gene/Protein Information For APIP (Source: Uniprot.org, NCBI)
Gene Name
APIP
Full Name
Methylthioribulose-1-phosphate dehydratase
Weight
27.125kDa
Superfamily
aldolase class II family
Alternative Names
APAF1 interacting protein; APAF1-interacting protein; APIP2; CGI29; CGI-29; dJ179L10.2; EC 4.2.1.109; Mmrp19; MTRu-1-P dehydratase; probable methylthioribulose-1-phosphate dehydratase APIP APIP2, CGI-29, CGI29, MMRP19, hAPIP APAF1 interacting protein methylthioribulose-1-phosphate dehydratase|MTRu-1-P dehydratase|probable methylthioribulose-1-phosphate dehydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APIP, check out the APIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APIP Antibody (Monoclonal, ABM19C7) (M09029)
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No publications found for M09029
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-APIP Antibody (Monoclonal, ABM19C7)
Question
We are currently using anti-APIP antibody (Monoclonal, ABM19C7) M09029 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
S. Carter
Verified customer
Asked: 2013-01-16
Answer
The anti-APIP antibody (Monoclonal, ABM19C7) (M09029) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-16