Product Info Summary
| SKU: | M09029 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB, FACS |
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Product info
Product Name
Anti-APIP Antibody (Monoclonal, ABM19C7)
SKU/Catalog Number
M09029
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-APIP Antibody (Monoclonal, ABM19C7) (Catalog# M09029). Tested in WB, FACS application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-APIP Antibody (Monoclonal, ABM19C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09029)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABM19C7
Isotype
IgG2b, Kappa
Immunogen
Full length recombinant protein of APIP
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M09029 is reactive to APIP in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.1 kDa
Background of APIP
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09029 is guaranteed for WB, FACS Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 4-6 µg/ml
FACS: 0.5-1 µg/10^6
Validation Images & Assay Conditions
Click image to see more details
Intracellular flow cytometric analysis of APIP in HeLa cells using 0.5 &mu
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Western blot analysis of APIP. Anti-APIP antibody (Clone: ABM19C7) was used at 4 &mu
Specific Publications For Anti-APIP Antibody (Monoclonal, ABM19C7) (M09029)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-APIP Antibody (Monoclonal, ABM19C7)
Question
We are currently using anti-APIP antibody (Monoclonal, ABM19C7) M09029 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
S. Carter
Verified customer
Asked: 2013-01-16
Answer
The anti-APIP antibody (Monoclonal, ABM19C7) (M09029) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-16

