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Product Info Summary
| SKU: | A03684 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-WAPL Antibody
SKU/Catalog Number
A03684
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WAPL Antibody (Catalog # A03684). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WAPL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03684)
Host
Rabbit
Contents
WAPL antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL. The immunogen is located within amino acids 30 - 80 of WAPL.
Cross-reactivity
WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Reactive Species
A03684 is reactive to WAPAL in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
132.9 kDa
Background of WAPAL
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03684 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.
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Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.
Specific Publications For Anti-WAPL Antibody (A03684)
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