Anti-GAPDHS Purified Monoclonal Antibody

Product Name

Anti-GAPDHS Purified Monoclonal Antibody

View all GAPDHS Antibodies

SKU/Catalog Number

M06121

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GAPDHS Purified Monoclonal Antibody (Catalog# M06121). Tested in WB, ICC, Flow Cytometry application(s). This antibody reacts with Pig, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-GAPDHS Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06121)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

Hs-8

Isotype

Mouse IgM

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Cross-reactivity

Cross-reactivity with CLIC5b was not determined.

Reactive Species

M06121 is reactive to GAPDHS in Human, Pig

Observed Molecular Weight

42 kDa

Calculated molecular weight

44.5 kDa

Background of GAPDHS

GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06121 is guaranteed for Flow Cytometry, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: 10 μg/ml; membrane permeabilization (acetone) is essential.
Flow cytometry: 3-12 µg/ml. Intraacrosomal staining.

Validation Images & Assay Conditions

See full target information GAPDHS

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