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Product Info Summary
| SKU: | PB9878 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-NAP2/PPBP Antibody Picoband®
SKU/Catalog Number
PB9878
PB0919 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAP2/PPBP Antibody Picoband® catalog # PB9878. Tested in ELISA(Cap), IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NAP2/PPBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9878)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived humn NAP2 recombinant protein (Position: S55-D128).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9878 is reactive to PPBP in Human
Observed Molecular Weight
19 kDa
Calculated molecular weight
13.9 kDa
Background of PPBP
Chemokine (C-X-C motif) ligand 7 (CXCL7), also known as NAP2 or Pro-Platelet basic protein (PPBP), is a human gene. The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Furthermore, the protein is an antimicrobial protein with bactericidal and antifungal activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9878 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA(Cap) , 1-5μg/ml, Human, -
Positive Control
WB: MCF-7 whole cell
IHC: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of NAP2 using anti-NAP2 antibody (PB9878).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP2 antigen affinity purified polyclonal antibody (Catalog # PB9878) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP2 at approximately 19 kDa. The expected band size for NAP2 is at 14 kDa.
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IHC analysis of NAP2 using anti-NAP2 antibody (PB9878).
NAP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NAP2 Antibody (PB9878) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Sandwich ELISA - Recombinant human CXCL7 protein standard curve.
Use in combination with reagents from Human CXCL7 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0729).
Specific Publications For Anti-NAP2/PPBP Antibody Picoband® (PB9878)
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Customer Q&As
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7 Customer Q&As for Anti-NAP2/PPBP Antibody Picoband®
Question
I see that the anti-NAP2/PPBP antibody PB9878 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-NAP2/PPBP antibody PB9878 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The anti-NAP2/PPBP antibody (PB9878) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
Question
Is this PB9878 anti-NAP2/PPBP antibody reactive to the isotypes of PPBP?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The immunogen of PB9878 anti-NAP2/PPBP antibody is E. coli-derived humn NAP2 recombinant protein (Position: S55-D128). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
I was wanting to use your anti-NAP2/PPBP antibody for WB for human peripheral blood monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood monocyte identification?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It shows on the product datasheet, PB9878 anti-NAP2/PPBP antibody has been validated for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
I have a question about product PB9878, anti-NAP2/PPBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9878 anti-NAP2/PPBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-27
Question
Would anti-NAP2/PPBP antibody PB9878 work for WB with peripheral blood monocyte?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
According to the expression profile of peripheral blood monocyte, PPBP is highly expressed in peripheral blood monocyte. So, it is likely that anti-NAP2/PPBP antibody PB9878 will work for WB with peripheral blood monocyte.
Boster Scientific Support
Answered: 2018-08-14
Question
Would PB9878 anti-NAP2/PPBP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
You can see on the product datasheet, PB9878 anti-NAP2/PPBP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-06
