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Product Info Summary
| SKU: | P06819-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, WB, Luminex |
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Product info
Product Name
Anti-Phospho-Histone H3 (Thr6) H3F3A Rabbit Monoclonal Antibody, Clone#RM160
SKU/Catalog Number
P06819-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H3 (Thr6) H3F3A Rabbit Monoclonal Antibody, Clone#RM160 (Catalog # P06819-2). Tested in WB, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H3 (Thr6) H3F3A Rabbit Monoclonal Antibody, Clone#RM160 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06819-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM160
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to Phospho-Histone H3 (Thr6)
Cross-reactivity
This antibody reacts to Histone H3 phosphorylated at Threonine 6. No cross-reactivity with other phosphorylated histones
Reactive Species
P06819-2 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06819-2 is guaranteed for ELISA, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.1 ug/mL - 1 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM160 at 0.1 ug/mL, showed a band of Histone H3 phospho-rylated at threonine 6 in HeLa cells.
Specific Publications For Anti-Phospho-Histone H3 (Thr6) H3F3A Rabbit Monoclonal Antibody, Clone#RM160 (P06819-2)
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