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Product Info Summary
| SKU: | M06819-20 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, WB, Luminex |
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Product info
Product Name
Anti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168
SKU/Catalog Number
M06819-20
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 (Catalog # M06819-20). Tested in WB, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-20)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM168
Isotype
Rabbit IgG
Immunogen
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys18).
Cross-reactivity
This antibody reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross-reactivity with monomethylated Lysine 18 (K18me1) or trimethylated Lysine 18 (K18me3), or other methylation in histone H3.
Reactive Species
M06819-20 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-20 is guaranteed for ELISA, WB, Luminex Boster Guarantee
Recommend Dilution
WB: 0.2 ug/mL – 1 ug/mL
ELISA: 0.1 ug/mL - 0.5 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.
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Specificity Test result
RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
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Dot Blot result
A Peptide dotblot shows RM168 only reacts to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).
Specific Publications For Anti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 (M06819-20)
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