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Product Info Summary
| SKU: | PA2029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EIF2S1 Antibody Picoband®
SKU/Catalog Number
PA2029
BA3268 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EIF2S1 Antibody catalog # PA2029. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2S1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2029)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2029 is reactive to EIF2S1 in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
36.1 kDa
Background of EIF2S1
EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2029 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
| Immunohistochemistry (Paraffin-embedded Section) | 2-5μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (PA2029).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (StacEIF2S1g gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HeLa whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified polyclonal antibody (PA2029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 38 kDa. The expected band size for EIF2S1 is at 36 kDa.
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IHC analysis of EIF2S1 using anti-EIF2S1 antibody (PA2029).
EIF2S1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF2S1 Antibody (PA2029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-EIF2S1 Antibody Picoband® (PA2029)
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Customer Q&As
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7 Customer Q&As for Anti-EIF2S1 Antibody Picoband®
Question
Would anti-EIF2S1 antibody PA2029 work for WB with cervix carcinoma erythroleukemia?
J. Mitchell
Verified customer
Asked: 2020-05-01
Answer
According to the expression profile of cervix carcinoma erythroleukemia, EIF2S1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-EIF2S1 antibody PA2029 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-05-01
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-EIF2S1 antibody PA2029. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this PA2029 anti-EIF2S1 antibody reactive to the isotypes of EIF2S1?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The immunogen of PA2029 anti-EIF2S1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-08
Question
We are currently using anti-EIF2S1 antibody PA2029 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The anti-EIF2S1 antibody (PA2029) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-12
Question
Is a blocking peptide available for product anti-EIF2S1 antibody (PA2029)?
M. Carter
Verified customer
Asked: 2019-05-17
Answer
We do provide the blocking peptide for product anti-EIF2S1 antibody (PA2029). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-17
Question
Does PA2029 anti-EIF2S1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-15
Question
I was wanting to use your anti-EIF2S1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-05
