Human Adiponectin PicoKine® Quick ELISA Kit

Adiponectin/Acrp30 ELISA kit, 90-minute one-step protocol

Human Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: FEK0595
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Adiponectin PicoKine® Quick ELISA Kit

See all Adiponectin/Acrp30 products

SKU/Catalog Number

FEK0595

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Adiponectin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0595)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N244

Sensitivity

<60 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0595 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0595 is reactive to ADIPOQ in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Application Guarantee

FEK0595 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Adiponectin/Acrp30

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Kit Components

Catalog numberDescription QuantityVolume
FEK0595-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0595-STHuman Adiponectin/Acrp30 Standard 2 100 ng/tube
FEK0595-AHuman ADIPOQ Antibody A 1 3ml
FEK0595-BHuman ADIPOQ Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0380.1410.241 0.4330.8201.2451.5392.037

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3726120584992736461147849590
Standard deviation182.57940.522346.56236.991033.022578.68
CV (%)4.9%7.8%4.7%6.5%9%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13726381038493865381253.791.4%
Sample 21205812135116221035711543712.136.1%
Sample 349927481965275450855504331645.093.2%
*number of samples for each test n=16.

Gene/Protein Information For ADIPOQ (Source: Uniprot.Org, NCBI)

Gene Name

ADIPOQ

Full Name

Adiponectin

Weight

26.414kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADIPOQ, check out the ADIPOQ Infographic

ADIPOQ infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

FEK0595 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...

Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).

Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...

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2 Customer Q&As for Human Adiponectin PicoKine® Quick ELISA Kit

Question

To Technical Support: I would like to know if the Human Adiponectin PicoKine kit will also work on porcine, since I am looking for porcine-specific adiponectin ELISA.

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We have not tested porcine samples on Human Adiponectin PicoKine kit, we cannot give you information about its cross reactivity. However, antibodies in this kit are mouse monoclonal and the epitope recognized is aa 104-244 which is in the globular domain and the alignment between the immunogen sequence and pig sequence is about a 84% level of homology, thus it entails that there is a good chance that it will work in porcine samples but we cannot guarantee.

Boster Scientific Support

Answered: 2020-05-01

Question

Does Human Adiponectin PicoKine kit has a good salivary-plasmatic ratio?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The Human Adiponectin PicoKine kit plasma sample usually test around 6000 ng/ml, and saliva is around 2 ng/ml. Take note that the saliva tends to have a large difference between samples.

Boster Scientific Support

Answered: 2020-01-24

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