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Human Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. Cited in 4 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine|
|Sample Volume:||100μl per well|
|Assay Range:||1.56 ng/ml - 100 ng/ml|
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
|Product Name||Human Adiponectin PicoKine™ Quick ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml.|
|Cite This Product||Human Adiponectin PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0595)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum, plasma (heparin, EDTA) and urine|
|Assay Range||1.56 ng/ml - 100 ng/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: E19-N244|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|Molecular Weight||26414 MW|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|FEK0595-CAP||Anti-tag Pre-coated 96-well Strip Microplate||1||12 strips of 8 wells|
|FEK0595-ST||Human Standard||2||100 ng/tube|
|FEK0595-C||Human ADIPOQ Antibody Cocktail||1||6ml|
|AR1106-7||TBS-T Wash Buffer (25x)||1||12ml|
|AR1104||Color Developing Reagent (TMB)||1||10ml|
|PLA-SEA||Adhesive Plate Sealers||2||Piece|
This data is generated from a recent batch of FEK0595 Human Adiponectin PicoKine™ Quick ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15min
We measured random samples of Human Adiponectin PicoKine™ Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Adiponectin PicoKine™ Quick ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway||PubMed ID 26305254|
|Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...||PubMed ID 29044029|
|Hatami Zargaran Z, Salehi M, Heydari St, Babajafari S. Int Cardiovasc Res J. 2014 Apr;8(2):52-6. Epub 2014 Apr 1. The Effects Of 6 Isocaloric Meals On Body Weight, Lipid Profiles, Leptin, And Adiponectin In Overweight Subjects (Bmi ≫ 25).||PubMed ID 24936481|
|Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...||PubMed ID 24986510|
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Comprehensive info on ADIPOQ, beautifully presented.Open Infographic
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