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Product Info Summary
| SKU: | A00241 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-H2AX pS139 H2AFX Antibody
SKU/Catalog Number
A00241
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-H2AX pS139 H2AFX Antibody (Catalog # A00241). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store Histone H2A.X (phospho S139) antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-H2AX pS139 H2AFX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00241)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.
Reactive Species
A00241 is reactive to H2AFX in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
15.1 kDa
Background of H2AFX
Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA is wrapped around histone-groups, consisting of the core histones H2A, H2B, H3 and H4. As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139, called gamma-H2AX. ATM, ATR and PRKDCs, kinases of the PI3-family, are responsible for this phosphorylation. The modification can happen accidentally during replication fork collapse, exogenous genotoxic agents, may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes, in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks, but is not required for the recruitment of proteins to the site of DSBs. Gamma-H2AX is a sensitive target for looking at DSBs in cells. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. The role of the phosphorylated form of the histone in DNA repair is under. Anti-H2AX pS139 is ideal for researched interested in Histones, DNA Damage and Repair, and Epigenetics.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00241 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000
WB: 1:1,000
Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to phosphorylated H2AX protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Validation Images & Assay Conditions
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Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 µg per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.
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Western Blot of Rabbit Anti-H2AX pS139 Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: HEK293T Whole cell Lysate [+]. Lane 3: MCF-7 Whole cell Lysate [+]. Lane 4: U-87-MG Whole cell Lysate [-]. Primary Antibody: Anti-H2AXpS139 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 min at RT. Block: Universal Fluorescent Buffer for 1hr at RT. Predicted MW: ~15kDa. Observed MW: ~17kDa. Exposure: 10sec.
Specific Publications For Anti-H2AX pS139 H2AFX Antibody (A00241)
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