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Product Info Summary
| SKU: | P14495 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194
SKU/Catalog Number
P14495
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (Catalog # P14495). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P14495)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM194
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to the Phospho-Histone H4 (pSer1).
Cross-reactivity
This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross-reactivity with Histone H2A phosphorylated at Serine 1.
Reactive Species
P14495 is reactive to HIST1H4A in Human, Vertebrates
Calculated molecular weight
11.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P14495 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Recommend Dilution
WB: 0.5 ug/mL - 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Specificity Test result
RM194 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
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ICC result
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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ICC result
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (P14495)
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1 Customer Q&As for Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194
Question
I would like using your anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal antibody, Clone#RM194 for amyloid fiber formation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
S. Krishna
Verified customer
Asked: 2016-11-18
Answer
I appreciate your inquiry. This P14495 anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal antibody, Clone#RM194 is validated on hela cells. It is guaranteed to work for ELISA, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-18
