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Product Info Summary
| SKU: | AZQ68SP3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish STAT5A Antibody Picoband®
SKU/Catalog Number
AZQ68SP3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish STAT5A Antibody Picoband® catalog # AZQ68SP3. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish STAT5A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ68SP3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish STAT5A recombinant protein (Position: D62-H726).
Reactive Species
AZQ68SP3 is reactive to STAT5A in Zebrafish
Background of STAT5A
Enables DNA binding activity and DNA-binding transcription factor activity. Acts upstream of or within several processes, including hemopoiesis; regulation of hemopoiesis; and regulation of primary metabolic process. Predicted to be located in cytosol. Predicted to be part of RNA polymerase II transcription regulator complex. Predicted to be active in cytoplasm and nucleus. Is expressed in adaxial cell; female organism; liver; and telencephalon. Used to study growth hormone insensitivity syndrome with immune dysregulation 1. Human ortholog(s) of this gene implicated in colon adenocarcinoma; colorectal adenocarcinoma; growth hormone insensitivity syndrome with immune dysregulation 1; and growth hormone insensitivity syndrome with immune dysregulation 2. Orthologous to several human genes including STAT5B (signal transducer and activator of transcription 5B).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ68SP3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of STAT5A using anti-STAT5A antibody (AZQ68SP3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified polyclonal antibody (AZQ68SP3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 87 kDa. The expected band size for STAT5A is at 87 kDa.
Specific Publications For Anti-Zebrafish STAT5A Antibody Picoband® (AZQ68SP3)
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