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Product Info Summary
| SKU: | FEK0724 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit
See all CXCL1/GRO alpha/KC/CINC-1 products
SKU/Catalog Number
FEK0724
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat CXCL1/Gro Alpha in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0724)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A25-K96
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0724 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0724 is reactive to Cxcl1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA)
Application Guarantee
FEK0724 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL1/GRO alpha/KC/CINC-1
Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0724-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0724-ST | Rat CXCL1/GRO alpha/KC/CINC-1 Standard | 2 | 10 ng/tube |
| FEK0724-A | Rat Cxcl1 Antibody A | 1 | 3ml |
| FEK0724-B | Rat Cxcl1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.035 | 0.124 | 0.201 | 0.358 | 0.640 | 1.121 | 1.863 | 2.448 |
Data Example Images
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Rat CXCL1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 25 | 171 | 426 | 22 | 156 | 411 |
| Standard deviation | 1.35 | 10.77 | 19.17 | 1.36 | 9.05 | 28.36 |
| CV(%) | 5.4% | 6.3% | 4.5% | 6.2% | 5.8% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL1/GRO alpha/KC/CINC-1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 25 | 23 | 41 | 35 | 31 | 1.67 | 5.4% |
| Sample 2 | 171 | 180 | 188 | 202 | 185 | 5.93 | 3.2% |
| Sample 3 | 426 | 498 | 502 | 514 | 485 | 15.52 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For Cxcl1 (Source: Uniprot.Org, NCBI)
Gene Name
Cxcl1
Full Name
Growth-regulated alpha protein
Weight
11.301kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha CXCL1 FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 C-X-C motif chemokine ligand 1 growth-regulated alpha protein|C-X-C motif chemokine 1|GRO-alpha(1-73)|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|MGSA alpha|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|melanoma growth stimulating activity, alpha|melanoma growth stimulatory activity alpha|neutrophil-activating protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cxcl1, check out the Cxcl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat CXCL1/Gro Alpha PicoKine® Quick ELISA Kit (FEK0724)
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