Rat CXCL1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

CXCL1/GRO alpha/KC/CINC-1 ELISA antibody pairs, Rat, for 5 plates

Rat CXCL1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Cxcl1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ0724
Size: For 5 plates, 96 wells each
Reactive Species: Rat
Application: ELISA

Product Name

Rat CXCL1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all CXCL1/GRO alpha/KC/CINC-1 products

SKU/Catalog Number

EZ0724

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Rat CXCL1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Cxcl1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat CXCL1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0724)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-K96

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0724 is reactive to Cxcl1 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EZ0724 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0724-CA Rabbit anti- rat Cxcl1 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0724-DA Biotinylated goat anti- rat Cxcl1 polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EZ0724-ST Lyophilized recombinant rat Cxcl1 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0440.130.2060.3760.6461.0881.6462.101

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)75.422154698.4285534
Standard deviation4.113.728.4620.5231.5
CV (%)5.4%6.2%5.2%6.1%7.2%5.9%

Gene/Protein Information For Cxcl1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14095

Gene ID

81503

Gene Name

Cxcl1

Full Name

Growth-regulated alpha protein

Weight

10.249kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha Cxcl1|CINC-1, Gro1|C-X-C motif chemokine ligand 1|growth-regulated alpha protein|C-X-C motif chemokine 1|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|cytokine-induced neutrophil chemoattractant 1|gro|platelet-derived growth factor-inducible protein KC

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cxcl1, check out the Cxcl1 Infographic

Cxcl1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cxcl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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