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Product Info Summary
|Description:||Transient overexpression lysate of TNF receptor-associated factor 7 (TRAF7)|
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TRAF7 (NM_032271) Human Over-expression Lysate
Transient overexpression lysate of TNF receptor-associated factor 7 (TRAF7)
Storage & Handling
Cite This Product
TRAF7 (NM_032271) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084231)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TRAF7 Human Over-expression Lysates NM_032271.
Western blot validation of overexpression lysate (LS084231) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TRAF7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TRAF7 (Source: Uniprot.org, NCBI)
E3 ubiquitin-protein ligase TRAF7
WD repeat TRAF7 family
DKFZp586I021; E3 ubiquitin-protein ligase TRAF7; EC 6.3.2.-; MGC7807; RFWD1RING finger protein 119; RNF119RING finger and WD repeat-containing protein 1; TNF receptor-associated factor 7ring finger and WD repeat domain 1 TRAF7 CAFDADD, RFWD1, RNF119 TNF receptor associated factor 7 E3 ubiquitin-protein ligase TRAF7|RING finger and WD repeat-containing protein 1|RING finger protein 119|RING-type E3 ubiquitin transferase TRAF7|TNF receptor-associated factor 7, E3 ubiquitin protein ligase|ring finger and WD repeat domain 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on TRAF7, check out the TRAF7 Infographic
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No publications found for LS084231
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