TRAF7 (NM_032271) Human Over-expression Lysate

Lysate of TRAF7 over-expression cell lines, WB control

Transient overexpression lysate of TNF receptor-associated factor 7 (TRAF7)

Product Info Summary

SKU: LS084231
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of TNF receptor-associated factor 7 (TRAF7)

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Product Name

TRAF7 (NM_032271) Human Over-expression Lysate

See all TRAF7 products

SKU/Catalog Number

LS084231

Size

100 µg

Description

Transient overexpression lysate of TNF receptor-associated factor 7 (TRAF7)

Storage & Handling

Cite This Product

TRAF7 (NM_032271) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084231)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for TRAF7 (Source: Uniprot.org, NCBI)

Uniprot ID

Q6Q0C0

Gene ID

84231

Gene Name

TRAF7

Full Name

E3 ubiquitin-protein ligase TRAF7

Weight

74.609kDa

Superfamily

WD repeat TRAF7 family

Alternative Names

DKFZp586I021; E3 ubiquitin-protein ligase TRAF7; EC 6.3.2.-; MGC7807; RFWD1RING finger protein 119; RNF119RING finger and WD repeat-containing protein 1; TNF receptor-associated factor 7ring finger and WD repeat domain 1 TRAF7 CAFDADD, RFWD1, RNF119 TNF receptor associated factor 7 E3 ubiquitin-protein ligase TRAF7|RING finger and WD repeat-containing protein 1|RING finger protein 119|RING-type E3 ubiquitin transferase TRAF7|TNF receptor-associated factor 7, E3 ubiquitin protein ligase|ring finger and WD repeat domain 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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