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Product Info Summary
| SKU: | A01687 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-14-3-3 epsilon YWHAE Antibody
SKU/Catalog Number
A01687
Size
100μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 epsilon YWHAE Antibody catalog # A01687. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 epsilon YWHAE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01687)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 epsilon. AA range:206-255
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01687 is reactive to YWHAE in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01687 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using 14-3-3 ε Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200
Specific Publications For Anti-14-3-3 epsilon YWHAE Antibody (A01687)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-14-3-3 epsilon YWHAE Antibody
Question
Is there a BSA free version of anti-14-3-3 epsilon antibody A01687 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-14-3-3 epsilon antibody A01687 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-14-3-3 epsilon antibody for WB for rat melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat melanoma identification?
P. Evans
Verified customer
Asked: 2019-06-20
Answer
You can see on the product datasheet, A01687 anti-14-3-3 epsilon antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-14-3-3 epsilon antibody A01687. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-30
