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Product Info Summary
| SKU: | A01687-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-14-3-3 Epsilon (D238) YWHAE Antibody
SKU/Catalog Number
A01687-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-14-3-3 Epsilon (D238) YWHAE Antibody catalog # A01687-2. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 Epsilon (D238) YWHAE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01687-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 ε.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01687-2 is reactive to YWHAE in Human, Mouse, Rat
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01687-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of 14-3-3 ε (D238) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of 14-3-3 ε (D238) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of 14-3-3 ε (D238) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-14-3-3 Epsilon (D238) YWHAE Antibody (A01687-2)
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