Show Order Info
Pack Size:100μg/vial
Validated Species:Human, Mouse, Rat
Application:IHC, ICC, WB
Data & Images


Product Name Anti-14-3-3 Sigma Antibody
SKU/Catalog Number PA1797
Description Rabbit IgG polyclonal antibody for 14-3-3 protein sigma(SFN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-14-3-3 Sigma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1797)
Replacement Item This antibody may replace the following items: sc-100638|sc-1019|sc-109424|sc-109425|sc-166473|sc-166726|sc-7681|sc-7683|sc-98414 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SFN
Protein Name 14-3-3 protein sigma
Molecular Weight 27774 MW
Protein Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. .
Tissue Specificity Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Subcellular Localization Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway.
Uniprot ID P31947
Alternative Names 14-3-3 protein sigma;Epithelial cell marker protein 1;Stratifin;SFN;HME1;
Research Areas |neuroscience|neurology process|neural signal transduction| signal transduction|adapters|cytoplasmic| neuroscience|cell type marker|neuron marker|synapse marker| stem cells|signaling pathways|wnt|14-3-3|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for 14-3-3 protein sigma

Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Anti-14-3-3 Sigma Antibody Images

Click the images to enlarge.

Anti-14-3-3 Sigma Antibody
Anti-14-3-3 sigma antibody, PA1797, Western blotting
All lanes: Anti 14-3-3 sigma (PA1797) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
Anti-14-3-3 Sigma Antibody
Anti-14-3-3 sigma antibody, PA1797, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-14-3-3 Sigma Antibody
Anti-14-3-3 sigma antibody, PA1797, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-14-3-3 Sigma Antibody
Anti-14-3-3 sigma antibody, PA1797, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
Write a review for PA1797


Xu Lx, Li Zh, Tao Yf, Li Rh, Fang F, Zhao H, Li G, Li Yh, Wang J, Feng X, Pan J. J Exp Clin Cancer Res. 2014 Dec 19;33:108. Doi: 10.1186/S13046-014-0108-3. Histone Acetyltransferase Inhibitor Ii Induces Apoptosis In Glioma Cell Lines Via The P53 S...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to er antibody, mkrn3 antibody