Anti-Hamartin/TSC1 Antibody Picoband™

TSC1 antibody

Boster Bio Anti-Hamartin/TSC1 Antibody Picoband™ catalog # PB9120. Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB9120
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Hamartin/TSC1 Antibody Picoband™

View all TSC1 Antibodies

SKU/Catalog Number

PB9120

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hamartin/TSC1 Antibody Picoband™ catalog # PB9120. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hamartin/TSC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9120)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9120 is reactive to TSC1 in Human, Rat

Applications

PB9120 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

129.767kDa

Background of TSC1

Hamartin also known as tuberous sclerosis 1 is a protein that in humans is encoded by the TSC1 gene. It is mapped to 9q34.13. This peripheral membrane protein was implicated as a tumor suppressor. It forms a complex with TSC2 that regulates mTORC1 signaling and may be also involved in vesicular transport and docking. Hamartin and TSC2 have critical roles in neuronal polarity, and that a common pathway regulates polarization and growth in neurons and cell size in other tissues. Hamartin is a growth inhibitory protein whose biologic effect is probably dependent on its interaction with tuberin. It also can affect cell proliferation via deregulation of G1 phase. Loss or perturbation of Hamartin function leads to loss of adhesion to the cellular matrix and initiates the development of TSC hamartomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For TSC1 (Source: Uniprot.org, NCBI)

Gene Name

TSC1

Full Name

Hamartin

Weight

129.767kDa

Alternative Names

Hamartin; KIAA0243MGC86987; LAMhamartin; TSC; TSC1; Tuberous sclerosis 1 protein; tuberous sclerosis 1; tumor suppressor TSC1 LAM, TSC TSC complex subunit 1 hamartin|truncated hemartin|tuberous sclerosis 1 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TSC1, check out the TSC1 Infographic

TSC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TSC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Hamartin/TSC1 Antibody Picoband™

Question

I have a question about product PB9120, anti-Hamartin/TSC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Kulkarni

Verified customer

Asked: 2020-04-15

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9120 anti-Hamartin/TSC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-15

Question

I am looking for using your anti-Hamartin/TSC1 antibody for negative regulation of neuron projection development studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

F. Li

Verified customer

Asked: 2020-02-14

Answer

We appreciate your inquiry. This PB9120 anti-Hamartin/TSC1 antibody is tested on rat brain tissue, tissue lysate, hela whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-14

Question

Is this PB9120 anti-Hamartin/TSC1 antibody reactive to the isotypes of TSC1?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The immunogen of PB9120 anti-Hamartin/TSC1 antibody is E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-13

Question

See attached the WB image, lot number and protocol we used for embryonic kidney using anti-Hamartin/TSC1 antibody PB9120. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-16

Question

I was wanting to use your anti-Hamartin/TSC1 antibody for WB for rat embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat embryonic kidney identification?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

It shows on the product datasheet, PB9120 anti-Hamartin/TSC1 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-17

Question

Is a blocking peptide available for product anti-Hamartin/TSC1 antibody (PB9120)?

N. Li

Verified customer

Asked: 2018-11-23

Answer

We do provide the blocking peptide for product anti-Hamartin/TSC1 antibody (PB9120). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-23

Question

We have observed staining in human blood. Do you have any suggestions? Is anti-Hamartin/TSC1 antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

Based on literature blood does express TSC1. Based on Uniprot.org, TSC1 is expressed in cerebellum, testis, bone marrow, embryonic kidney, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, peripheral blood, blood, blood lymphoblast, peripheral blood leukocyte, among other tissues. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-09-14

Question

Would PB9120 anti-Hamartin/TSC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-20

Answer

You can see on the product datasheet, PB9120 anti-Hamartin/TSC1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-20

Question

Is there a BSA free version of anti-Hamartin/TSC1 antibody PB9120 available?

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hamartin/TSC1 antibody PB9120 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-26

Question

I see that the anti-Hamartin/TSC1 antibody PB9120 works with WB, what is the protocol used to produce the result images on the product page?

S. Banerjee

Verified customer

Asked: 2017-07-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-13

Question

We are currently using anti-Hamartin/TSC1 antibody PB9120 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?

B. Wu

Verified customer

Asked: 2017-07-11

Answer

The anti-Hamartin/TSC1 antibody (PB9120) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-11

Question

you antibody to test anti-Hamartin/TSC1 antibody PB9120 on rat embryonic kidney for research purposes, then I may be interested in using anti-Hamartin/TSC1 antibody PB9120 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

T. Zhao

Verified customer

Asked: 2016-03-23

Answer

The products we sell, including anti-Hamartin/TSC1 antibody PB9120, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-03-23

Question

Would anti-Hamartin/TSC1 antibody PB9120 work for WB with embryonic kidney?

V. Rodriguez

Verified customer

Asked: 2015-08-04

Answer

According to the expression profile of embryonic kidney, TSC1 is highly expressed in embryonic kidney. So, it is likely that anti-Hamartin/TSC1 antibody PB9120 will work for WB with embryonic kidney.

Boster Scientific Support

Answered: 2015-08-04

Question

Will anti-Hamartin/TSC1 antibody PB9120 work on monkey WB with cervix carcinoma erythroleukemia?

B. Anderson

Verified customer

Asked: 2014-06-16

Answer

Our lab technicians have not validated anti-Hamartin/TSC1 antibody PB9120 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Hamartin/TSC1 antibody PB9120 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-06-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Hamartin/TSC1 antibody PB9120. Let me know if you need anything else.

B. Brown

Verified customer

Asked: 2013-10-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-28

Question

My colleagues were content with the WB result of your anti-Hamartin/TSC1 antibody. However we have been able to see positive staining in bone marrow cytoplasm. using this antibody. Is that expected? Could you tell me where is TSC1 supposed to be expressed?

L. Carter

Verified customer

Asked: 2013-01-10

Answer

Based on literature, bone marrow does express TSC1. Generally TSC1 expresses in cytoplasm. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-01-10

Order DetailsPrice
PB9120

100μg

$370
PB9120-10ug

10μg sample (liquid)

$99
PB9120-Biotin

100 μg Biotin conjugated

$570
PB9120-Cy3

100 μg Cy3 conjugated

$570
PB9120-Dylight488

100 μg Dylight488 conjugated

$570
PB9120-Dylight550

100 μg Dylight550 conjugated

$570
PB9120-Dylight594

100 μg Dylight594 conjugated

$570
PB9120-FITC

100 μg FITC conjugated

$570
PB9120-HRP

100 μg HRP conjugated

$570
PB9120-APC

100 μg APC conjugated

$670
PB9120-PE

100 μg PE conjugated

$670
PB9120-carrier-free

Carrier Free

$370

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