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Product Info Summary
| SKU: | A03696 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-40S ribosomal protein S14 RPS14 Antibody
SKU/Catalog Number
A03696
Size
100ul
Form
Liquid
Description
Boster Bio Anti-40S ribosomal protein S14 RPS14 Antibody catalog # A03696. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-40S ribosomal protein S14 RPS14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03696)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human RPS14(NP_005608.1).
Reactive Species
A03696 is reactive to RPS14 in Human, Mouse, Rat
Calculated molecular weight
16.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03696 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using RPS14 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunofluorescence analysis of MCF7 cells using RPS14 antibody . Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat pancreas using RPS14 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded mouse spinal cord using RPS14 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Specific Publications For Anti-40S ribosomal protein S14 RPS14 Antibody (A03696)
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