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Product Info Summary
| SKU: | A02061 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-A-Raf Antibody
SKU/Catalog Number
A02061
Size
100μl
Form
Liquid
Description
Boster Bio Anti-A-Raf Antibody catalog # A02061. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A-Raf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02061)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:276-325
Reactive Species
A02061 is reactive to ARAF in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
67.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02061 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis in MCF-7 cells transfected with siA-Raf.
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Western Blot analysis of various cells using A-Raf Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-A-Raf Antibody (A02061)
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Customer Q&As
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4 Customer Q&As for Anti-A-Raf Antibody
Question
Is this A02061 anti-A-Raf antibody reactive to the isotypes of ARAF?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of A02061 anti-A-Raf antibody is Synthesized peptide derived from human A-Raf around the non-phosphorylation site of Y302. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
Does anti-A-Raf antibody A02061 work on feline WB with kidney?
C. Johnson
Verified customer
Asked: 2019-10-02
Answer
Our lab technicians have not tested anti-A-Raf antibody A02061 on feline. You can run a BLAST between feline and the immunogen sequence of anti-A-Raf antibody A02061 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-02
Question
you antibody to test anti-A-Raf antibody A02061 on rat leukemic t-cell for research purposes, then I may be interested in using anti-A-Raf antibody A02061 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
The products we sell, including anti-A-Raf antibody A02061, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-11
Question
I was wanting to use your anti-A-Raf antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?
M. Brown
Verified customer
Asked: 2013-11-28
Answer
It shows on the product datasheet, A02061 anti-A-Raf antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-28
