Product Info Summary
SKU: | PB9020 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Serum Amyloid P/APCS Antibody Picoband™
View all Pentraxin 2/SAP Antibodies
SKU/Catalog Number
PB9020
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Serum Amyloid P/APCS Antibody Picoband™ catalog # PB9020. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Serum Amyloid P/APCS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9020)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Serum Amyloid P recombinant protein (Position: Q21-D224). Mouse Serum Amyloid P shares 70% and 80% amino acid (aa) sequences identity with human and rat Serum Amyloid P, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9020 is reactive to APCS in Mouse, Rat
Applications
PB9020 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
25-29 kDa
Calculated molecular weight
25.387kDa
Background of Pentraxin 2/SAP
Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Mouse, -
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB9020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25-29 kDa. The expected band size for Serum Amyloid P is at 25 kDa.
Click image to see more details
Figure 2. IHC analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9020).
Serum Amyloid P was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Serum Amyloid P Antibody (PB9020) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For APCS (Source: Uniprot.org, NCBI)
Gene Name
APCS
Full Name
Serum amyloid P-component
Weight
25.387kDa
Superfamily
pentraxin family
Alternative Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component APCS HEL-S-92n, PTX2, SAP amyloid P component, serum serum amyloid P-component|9.5S alpha-1-glycoprotein|epididymis secretory sperm binding protein Li 92n|pentaxin-related|pentraxin-2|pentraxin-related
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APCS, check out the APCS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Serum Amyloid P/APCS Antibody Picoband™ (PB9020)
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No publications found for PB9020
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