Anti-A-Raf (phospho-Y302) Antibody

Product Name

Anti-A-Raf (phospho-Y302) Antibody

View all A-RAF Antibodies

SKU/Catalog Number

A02061Y302

Size

100ul

Form

Liquid

Description

Boster Bio Anti-A-Raf (phospho-Y302) Antibody catalog # A02061Y302. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-A-Raf (phospho-Y302) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02061Y302)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human A-Raf around the phosphorylation site of S299.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02061Y302 is reactive to ARAF in Human, Mouse, Rat

Applications

A02061Y302 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

67.585kDa

Background of A-RAF

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

McKinnon C.M., Eur. J. Biochem. 169:547-553(1987).
Tosi M., Biochemistry 26:8516-8524(1987).
Kusumoto H., Proc. Natl. Acad. Sci. U.S.A. 85:7307-7311(1988).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-A-Raf (phospho-Y302) Antibody A02061Y302

Western blot (WB) analysis of p-ARAF (Y302) polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate treated with PMA(100nM

Gene/Protein Information For ARAF (Source: Uniprot.org, NCBI)

Alternative Names

ARaf; A-Raf; ARAF1A-RAF; EC 2.7.11; EC 2.7.11.1; Oncogene ARAF1; PKS; PKS2; PKS2A-Raf proto-oncogene serine/threonine-protein kinase; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene homolog ARAF A-RAF1, PKS2, RAFA1, ARAF A-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase A-Raf|A-Raf proto-oncogene serine/threonine-protein kinase|Oncogene ARAF1|Ras-binding protein DA-Raf|proto-oncogene A-Raf-1|proto-oncogene Pks|v-raf murine sarcoma 3611 viral oncogene homolog 1|v-raf murine sarcoma 3611 viral oncogene-like protein

For more info on ARAF, check out the ARAF Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ARAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ARAF Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis