Product Info Summary
SKU: | M05358 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ABCF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05358
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ABCF1 Rabbit Monoclonal Antibody catalog # M05358. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05358)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FGE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ABCF1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05358 is reactive to ABCF1 in Human, Rat
Applications
M05358 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
95.926kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ABCF1 expression in K562 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using ABCF1 Antibody.
Click image to see more details
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For ABCF1 (Source: Uniprot.org, NCBI)
Gene Name
ABCF1
Full Name
ATP-binding cassette sub-family F member 1
Weight
95.926kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC27; ABC50TNFalpha-inducible ATP-binding protein; ATP-binding cassette 50 (TNF-alpha stimulated); ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; ATP-binding cassette, sub-family F (GCN20), member 1; EC 2.7.7.6; EC 3.6.3.17; EST123147; TNF-alpha-stimulated ABC protein ABCF1 ABC27, ABC50 ATP binding cassette subfamily F member 1 ATP-binding cassette sub-family F member 1|ATP-binding cassette 50 (TNF-alpha stimulated)|ATP-binding cassette, sub-family F (GCN20), member 1|TNF-alpha-stimulated ABC protein|TNFalpha-inducible ATP-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCF1, check out the ABCF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCF1 Rabbit Monoclonal Antibody (M05358)
Hello CJ!
No publications found for M05358
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ABCF1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-ABCF1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-ABCF1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-ABCF1 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
As indicated on the product datasheet, M05358 anti-ABCF1 Rabbit Monoclonal antibody has been validated for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
Can you help my question with product M05358, anti-ABCF1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05358 anti-ABCF1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-ABCF1 Rabbit Monoclonal antibody M05358 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The anti-ABCF1 Rabbit Monoclonal antibody (M05358) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-02
Question
I see that the anti-ABCF1 Rabbit Monoclonal antibody M05358 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ABCF1 Rabbit Monoclonal antibody M05358. Let me know if you need anything else.
B. Moore
Verified customer
Asked: 2017-04-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-03
Question
We want to test anti-ABCF1 Rabbit Monoclonal antibody M05358 on human leukemic t-cell for research purposes, then I may be interested in using anti-ABCF1 Rabbit Monoclonal antibody M05358 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jones
Verified customer
Asked: 2016-12-02
Answer
The products we sell, including anti-ABCF1 Rabbit Monoclonal antibody M05358, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-02
Question
Will anti-ABCF1 Rabbit Monoclonal antibody M05358 work on zebrafish WB with cervix carcinoma erythroleukemia?
T. Walker
Verified customer
Asked: 2016-11-03
Answer
Our lab technicians have not tested anti-ABCF1 Rabbit Monoclonal antibody M05358 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ABCF1 Rabbit Monoclonal antibody M05358 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-03