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Product Info Summary
| SKU: | M05358 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ABCF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05358
BM4542 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ABCF1 Rabbit Monoclonal Antibody catalog # M05358. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05358)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FGE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ABCF1
Reactive Species
M05358 is reactive to ABCF1 in Human, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
95.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05358 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of ABCF1 expression in K562 cell lysate.
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Western blot analysis of ABCF1 using anti-ABCF1 antibody (M05358).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCF1 antigen affinity purified monoclonal antibody (M05358) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ABCF1 at approximately 110 kDa. The expected band size for ABCF1 is at 96 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using ABCF1 Antibody.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
Specific Publications For Anti-ABCF1 Rabbit Monoclonal Antibody (M05358)
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7 Customer Q&As for Anti-ABCF1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-ABCF1 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
As indicated on the product datasheet, M05358 anti-ABCF1 Rabbit Monoclonal antibody has been validated for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
Can you help my question with product M05358, anti-ABCF1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05358 anti-ABCF1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-ABCF1 Rabbit Monoclonal antibody M05358 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The anti-ABCF1 Rabbit Monoclonal antibody (M05358) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-02
Question
I see that the anti-ABCF1 Rabbit Monoclonal antibody M05358 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ABCF1 Rabbit Monoclonal antibody M05358. Let me know if you need anything else.
B. Moore
Verified customer
Asked: 2017-04-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-03
Question
We want to test anti-ABCF1 Rabbit Monoclonal antibody M05358 on human leukemic t-cell for research purposes, then I may be interested in using anti-ABCF1 Rabbit Monoclonal antibody M05358 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jones
Verified customer
Asked: 2016-12-02
Answer
The products we sell, including anti-ABCF1 Rabbit Monoclonal antibody M05358, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-02
Question
Will anti-ABCF1 Rabbit Monoclonal antibody M05358 work on zebrafish WB with cervix carcinoma erythroleukemia?
T. Walker
Verified customer
Asked: 2016-11-03
Answer
Our lab technicians have not tested anti-ABCF1 Rabbit Monoclonal antibody M05358 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ABCF1 Rabbit Monoclonal antibody M05358 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-03
